Question Give an account of how enzymes work with particular reference to factors that affect their activities. Answer All living things require catalysts to catalyse the reaction which they carry out to survive. Enzymes are the catalysts of biological systems without enzymes reactions in cells would occur all much to slow a rate and the organism would not survive. Most enzymes‚ with the exception of some RNA catalysts‚ are proteins. They often require a co factor such as zinc or iron‚ or a complete organic
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NaCl Effects on Peroxidase Activity My experiment was to see if adding NaCl to solution would have any effects on peroxidase activity. The materials that were used in this experiment were pH 7 buffer(DI water)‚ peroxidase‚ NaCl‚ guaiacol and hydrogen peroxide; added in that order. Blanks were created for each NaCl concentration‚ 0%‚ 5%‚ 7.5% and 10%. Each cuvette had .5ml of pH 7 buffer‚ 1ml of peroxidase‚ .02ml guaiacol for the experimental cuvettes and 0ml of guaiacol for the blank cuvettes‚
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Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic
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Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how the experiment was created
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Enzymes are catalytic proteins which speed up the rate of reactions. Every enzyme has a specific function – meaning‚ they can only bind to certain substrates. Because these enzymes are proteins‚ they can be denatured. Enzymes can be denatured by many factors‚ such as pH and temperature. This lab was divided into three parts which examined the effects of pH‚ enzyme concentration and temperature on the rate of which enzymes catalyze. The pH is an index of hydrogen ions. In acidic conditions‚ where
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Dependant - The intensity of the reaction. Constants – The temperature‚ the amount of liver‚ pH level‚ test time‚ Materials: Solutions of hydrogen peroxide varying in concentration 1‚2‚4‚6%‚ fresh liver(containing catalase)‚ sharp knife‚ forceps‚ cutting tile‚ 2x 10mL measuring cylinder‚ 1x 100ml measuring cylinder‚ 1x 50mL measuring cylinder‚ stop watch‚ detergent‚ teat pipette‚ distilled water. Method: 1) The materials for the practical were collected. 2) We set up a clean cutting area
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Temperature on the Enzyme Catalase Stephen Francis Biology 183 Abstract This experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature‚ 2°C‚ 50°C‚ and 60°C. The overall result of this experiment proved that this reaction
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20/04/2013 Pepsin Experiment Experiment protocol to test the effect of temperature on the enzyme pepsin Lab Day: Tuesday Lab Time: 10-1pm Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?: G20 Demonstrator Name: Tamsin Peters Names of Group Members: Roanna Humphries Daniel Markus Nicholle Cooke-Hayes Dean Do Rosario Pepsin Name of Enzyme You are Investigating: Table of Contents Table of Contents ........................
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LAB 1: What temperature does the enzyme actually work properly in? (Hypothesis) If the temperature is below 40 but above 20‚ then the liver will show bubbles. If the temperature is raised higher than the optimum temperature‚ then an extreme decline in enzyme activity would occur following by the quick denaturing of the enzyme‚ rendering it is permanently useless. Also about 37°C is body temperature. The liver that was at 25°C had a huge amount of bubbles (a 4 on the scale) and the 0°C
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Enzyme kinetics Purpose: The goal of this investigation was to measure the amounts of products made and see the different elements that that affect the rate of breakdown of p-Nitro phenol in the absence or presence of cellobiase….. Methods: Activity #1 The materials used for this activity are as follows: 1.5 mM substrate‚ enzyme‚ Stop solution‚ buffer‚ DPTPs‚ 15 ml conical tubes‚ cuvettes‚ marker‚ beaker‚ distilled water‚ spectrophotometer‚ stop watch. First four 15 ml conical tubes
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