Enzyme Catalase Lab Report
The Effect of Temperature on Enzyme Activity and Oxygen Production
Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how the experiment was created. As a result we found that the higher the temperature in the water bath the more oxygen will be produced and “rejected” from the reaction chamber. In conclusion, the reaction chamber placed in the highest temperature …show more content…
Obtain a small amount of stock catalase solution in a 50mL beaker. (Put on ice to keep catalase cool)
3. Obtain a reaction chamber and a number of filter paper disks. (4 for each trial)
4. Prepare 4 disks for use in the reaction chamber. Prepare each disk, one at a time, by holding each by its edge with a pair of forceps (tweezers) and dipping it into the stock catalase solution for a few seconds.
*** Stir your catalase solution (liver extract) before every disk is dipped. Remove the excess liquid from the disk by blotting the disk on a paper towel. With another paper towel dry the tip of the forceps, so it doesn’t drip in the next step.
5. Next, transfer the dampened disks to the top interior wall of the reaction vessel. The wet filter paper disks will stick to the side wall of the glass. Position the disks in the upper half of the reaction vessel (the half near the opening). Repeat this procedure with the other disks, placing all disks on the same surface of the reaction vessel.
* One person is each group should soak and handle all disks for all experiments. In this way, the techniques will remain similar and key operations will be performed consistently.
Place four catalase-soaked filter paper disks high on one interior sidewall of the reaction …show more content…
As temperature increased the amount of oxygen produced increased as well. In the tooth-pick lab students posed as enzymes and did various things to test what may affect the performance of an enzyme one of those things being temperature. In that lab students were asked to place their hands in an ice bath for a certain period of time (2 minutes I believe), in turn numbing our hands (the enzyme). The results were the same as what was predicted in our temperature experiment, the number of tooth-picks broken was dramatically decreased after having our hands in the ice bath. This has direct correlation to what we had tested in the procedure above. To better our experiment and get more accurate results we could have had more control over the temperature of the water bath, other than that the experiment created and tested had matched our hypothesis and had reasonable results that we could back up with scientific
Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how the experiment was created. As a result we found that the higher the temperature in the water bath the more oxygen will be produced and “rejected” from the reaction chamber. In conclusion, the reaction chamber placed in the highest temperature …show more content…
Obtain a small amount of stock catalase solution in a 50mL beaker. (Put on ice to keep catalase cool)
3. Obtain a reaction chamber and a number of filter paper disks. (4 for each trial)
4. Prepare 4 disks for use in the reaction chamber. Prepare each disk, one at a time, by holding each by its edge with a pair of forceps (tweezers) and dipping it into the stock catalase solution for a few seconds.
*** Stir your catalase solution (liver extract) before every disk is dipped. Remove the excess liquid from the disk by blotting the disk on a paper towel. With another paper towel dry the tip of the forceps, so it doesn’t drip in the next step.
5. Next, transfer the dampened disks to the top interior wall of the reaction vessel. The wet filter paper disks will stick to the side wall of the glass. Position the disks in the upper half of the reaction vessel (the half near the opening). Repeat this procedure with the other disks, placing all disks on the same surface of the reaction vessel.
* One person is each group should soak and handle all disks for all experiments. In this way, the techniques will remain similar and key operations will be performed consistently.
Place four catalase-soaked filter paper disks high on one interior sidewall of the reaction …show more content…
As temperature increased the amount of oxygen produced increased as well. In the tooth-pick lab students posed as enzymes and did various things to test what may affect the performance of an enzyme one of those things being temperature. In that lab students were asked to place their hands in an ice bath for a certain period of time (2 minutes I believe), in turn numbing our hands (the enzyme). The results were the same as what was predicted in our temperature experiment, the number of tooth-picks broken was dramatically decreased after having our hands in the ice bath. This has direct correlation to what we had tested in the procedure above. To better our experiment and get more accurate results we could have had more control over the temperature of the water bath, other than that the experiment created and tested had matched our hypothesis and had reasonable results that we could back up with scientific