Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.…
4. Potato contains plant cells with cellulose, which is rigid and rough. The blender allows us to break through the cellulose that forms the cell wall which subsequently allows us to access the enzymes.…
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In regards to peroxidase, several points can be made about the overall effects of these factors. Factors such as temperature and pH have been shown to have an effect on the performance of enzymes (Vishwanatha, K.S., Food Chemistry. 2: 402-407; 2009). Adjusting the temperature will produce one of two results; if the enzymatic reaction occurs and the enzyme is colder than the optimum temperature, fewer collisions between enzyme and substrate will occur thus reducing the rate of the reaction. On the other hand, if the enzyme has been heated to a higher temperature than the optimum but not high enough to denature the enzyme, more of these collisions will occur and the reaction rate should speed up (Table 2, Graph 2). The second of the common factors is pH. Everything in the world has extremes on each end of the spectrum where the body, cell, etc. stops reacting to its environment. This goes for enzymes as well. If a solution is too basic or too acidic in comparison to the enzymes optimum pH, the enzyme will begin to degrade until it eventually stops reacting all together. It is important that students and biologists understand these and other optimal conditions for enzymes to react in. For the pH aspect of the experiment the group predicted, through reading of the laboratory manual and general knowledge of enzyme properties, that the rate would vary based on both…
In this lab, I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than 37℃, then it will break down the peroxide faster because yeast tends to rise faster as the temperature increases. Some constants in this lab are: the 10 mL of peroxide, the 190 mL of water, the same timer, the same sheet of filter paper and the…
Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase, determine factors that influence how quickly the reaction takes place, and determine factors that influence how well enzymes function. The first test, we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added a pinch of sand into the test tube. For the first test tube we observed that the hydrogen peroxide did not react with the sand because there is no catalase in the sand. Our time for the reaction was zero seconds.…
In this laboratory exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.…
Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish, one whole and one ground up, which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined by observing a larger formation of bubbles, the visual indicator of hydrogen peroxide being broken down into a non-toxic form of hydrogen and water. This study allowed us to observe first hand catalase can perform further when the surface area is larger.…
Abstract: This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won't denature in cold temperatures, but have slowed molecular activity. pH will also have an affect on an enzymes efficiency, when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable after the experiment was complete.…
The purpose of this investigation is to figure out how temperature, pH, and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to occur, allowing vital processes such as digestion, ATP production, and DNA replication to occur efficiently. Using a Vernier Gas Pressure Sensor the rate of reaction of the enzyme catalase as it decomposed Hydrogen Peroxide in a test tube was measured and then results were put on the screen of a Macintosh computer using Logger Pro software and Vernier computer software. Several trials were then done with one factor, either temperature, pH, or enzyme concentration being altered, while the remaining two stayed constant. The rate of reaction of catalase should increase with enzyme concentration until a certain point until it reaches its limit. For temperature the rate of reaction of catalase should increase until the enzyme catalase denatures, and then after the rate should start to decrease. When exposed to different pH levels the rate of reaction of catalase should increase as the enzyme catalase reaches its optimal pH level, once it’s at that point the rate of reaction should then decrease as the pH level goes farther away from catalase’s optimal pH level. The data for the trials of pH and enzyme concentration support the expected hypotheses of how the reaction rate would be affected, however the data did not support the hypothesis made for the effects of temperature on an enzyme’s reaction rate due to divergent data from the expected results. (need this line or is conclusion and results of data and trends summarized with data supported hypotheses) ask if titel too long,,…
Aim: To investigate the Effect of temperature (10, 37, 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3)…
The mistake made throughout this experiment was the addition of 15-mL of the 1.5% H2O2 substrate instead of the 10-mL directed. This mistake led to the chemical reaction occurring at a slower rate than it would have if the proper amount was added. The more substrate that is present, the more of the enzyme that is required, so because there was more of the H2O2 substrate present, the enzyme (catalase solution) was not as effective.…
The enzyme catalase worked best under the controlled condition, it had the most significant visible release of energy. When it was placed in the hot water bath, the enzyme stopped functioning at optimum level…
Temperature can also denature or effect the reaction rate of the enzyme. As you increase temperature the various bonds that help made the 3-D tertiary structure start to break apart as they absorb more energy. This also causes the active site of the enzyme to change shape and reduce the reaction rate. On the other hand, as you decrease temperature the substrates won’t come into contact with the active site of the enzyme as much and will reduce the reaction rate. Most enzymes have an optimal pH, temperature, and salinity. At the optimal levels the enzyme should perform at its fastest reaction rate possible. In our lab, the enzyme(catalase) performs at optimal level of pH 7. As you change the pH of the solution(independent variable) in which the enzyme resides in you will see a decrease in the reaction rate of the enzyme(dependent variable). You can measure this reaction rate by looking at how much oxygen is being released and calculating the rate of increase in(O2%/min). You will test three different pH levels in this lab which are pH 4, pH 7, and pH 10. You will have three trials for each level of treatment to gather accurate…
Enzymes lower the amount of energy needed in a chemical reaction. This happens because enzymes are catalysts, so they speed up the activation rates that occur in living organisms. Without enzymes, it would be difficult to break down particles like food in the digestion system. Enzymes are all very specific to what chemical reactions they will work with, and the temperature, pH, and salt concentration have to be a specific levels in order for the enzyme to function. The structure of each enzyme has a fixed shape in which a certain the substrate will fit into.…