Conclusion 7 8.0 Appendix 8 9.0 Reference List 10 1.0 Abstract Enzymes are catalysts to ensure homeostasis in the body [2]. Homeostasis is the tendency to maintain the internal environment [2]. Hydrogen peroxide is an oxidizing agent that can be decomposed by catalase [11]‚ the liver is able to decompose hydrogen peroxide enzymatically because it contains catalase [12]. The reaction rate of an enzyme catalysed reaction under the effect of changes in temperature was tested in this
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structure of the enzyme is mainly dependent on the active site and variable groups. Extreme temperatures or extreme pHs can alter the structure of an enzyme. Enzymes function to lower the activation energy to break the bonds. They achieve this by putting stress and pressure on the bonds or creating a microenvironment for the substrate. Enzymes are regulated by inhibitors or activators and can be inhibited by the products of the reaction‚ called feedback inhibition. Enzymes are catalytic proteins;
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Investigating the Enzymatic Activity of Catecholase through Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration University of Alabama at Birmingham Burgess‚ B.N. Introduction: Background Enzymes are macromolecules that act as catalysts in living organisms by speeding up chemical reactions without being changed or destroyed by the reaction (Campbell and Reece‚ 2008). Enzymes are able to speed up the rate of a chemical reaction by decreasing the activation energy during the reaction
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Enzymes Enzymes are… * Biological catalysts Lower the energy level needed for a biochemical reaction to occur. This energy level is called activation energy. * Proteins Polypeptide chains made up of 100’s-1000’s of amino acids in a specific sequence. * Do not get “used up” in a reaction The number of “uses” of an enzyme depends on the enzyme. * Work more efficiently at certain optimum temperatures. * They are “reaction-specific”. Each enzyme is included in one reaction.
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
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If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH nullifies
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Enzymes Introduction This study allows the investigation of enzymatic reactions behavior. An enzyme is a protein catalyst reaction by lowering the activation energy required for that reaction. The enzyme is unaltered at the completion of the reaction. In this stimulation the amount of product produced during the course of an enzymatic product produced during the course of an enzymatic reaction will be measured. Hypothesis 1: What is the estimate optimal of ph? Hypothesis 2: What is the estimate optimal of temperature
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Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
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observation that each parent gives one allele for each trait at random to each offspring‚ regardless of whether the allele is expressed. The segregation of chromosomes at random during anaphase I explains Mendel’s observation that factors‚ or genes‚ for different traits are inherited independently of each other. Apply the rules of probability to infer genotypes from test crosses A test cross is used to determine the genotype of an individual with a dominant trait. Because the trait is dominant‚ an individual
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MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment‚ we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly‚ moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water‚ distilled water‚ Soda and milk as the main
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