when the enzyme and buffer in the assay are held constant were experimented. We analyzed the change in absorbencies over time for varying substrate concentrations. There were four experimental assays which contained 1% enzyme solution‚ substrate solution of 0%‚ 1%‚ 2%‚ and 3% concentrations‚ guaiacol‚ and pH 7 buffer. At 2% concentration there was a greater enzymatic activity and at 3% concentration enzymatic activity decreased. The results were supported of our hypothesis. INTRODUCTION Enzymes are proteins
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Institute Physics Course 2014 - Rocket Lab Report Title: RockSim and Electronic Altimeter Measurement Techniques Name: Scarlet Henriquez Email: scarletpatricia94@aol.com LAB SESSION ___8____Assignment _____7_____ Due Date: ___7/8/14______ Objective: Launch a smaller rocket to learn how to compare RockSim predictions (computer based) to an actual instrument measurement carried by the rocket (Electronic Altimeter). This Lab shows how better the electronic measurement
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Bio 205 Lab W/8:00 Enzyme II Write-Up Methods: My partner and I ran two experiments to measure the activity of the enzyme horseradish peroxidase under varied conditions. The first of which measured the effects of altered pH levels‚ while the goal of the second was to examine the effects of varied temperatures. To test the effects of pH on horseradish peroxidase‚ we began by zeroing a Spec 20 with 5.0mL of substrate (25mM guiacol) at pH 6.5. Once the Spec 20 was accurately zeroed‚ we added 100μL
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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Lab Report 3 In this lab‚ we will take a trip to the planetarium lab and will learn about Right ascension and declination‚ and altitude and zenith. After looking at the different points shown‚ we will log the altitude and zenith in the chart in our lab manual. Now we will look at the same points and label the right ascension and declination. Then we will learn about the easiest way to locate the star Polaris. As we started the first program‚ we answered a few questions to make sure we knew
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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This lab is adapted from the canned denatured pineapple experiment. Instead of investigating the effect of canning on enzyme activity‚ we look for the presence of the substrate. This is a useful teaching lab for all the criteria and requires very few resources. Background Information: Gelatin is made from a protein called collagen which comes from the joints of animals. Gelatin may be dissolved in hot water. As the dissolved gelatin mixture cools‚ the collagen forms into a matrix that
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This lab report will be detailing the steps taken and the results discovered when using spectrophotometry to determine the percentage of copper in a copper-clad penny and the thicknes of the copper layer on the copper-clad penny. After 1982‚ copper coating has been used in the creation of the penny because the cost of pure copper has increase to the point that the amount needed t omake a penny cost far more than the actual value of the penny. This lab allowed us to see just how much copper coating
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Effect of enzyme concentration on rate of reaction Research question: is there a relationship between catalase concentration and the rate of reaction? Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled
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