and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
Premium Enzyme Catalysis Chemistry
effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower rate for the enzyme reaction
Premium Chemical reaction Enzyme Enzyme
carefully‚ as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful‚ ensuring they don’t come into contact with our eyes‚ skin or clothes. Catalyse is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and Oxygen. We will be measuring the amount of Oxygen released from the Hydrogen Peroxide. In
Premium Hydrogen peroxide Oxygen Chemical reaction
Enzymes are important components of life‚ facilitating reactions that are necessary for an organism to live. Enzymes can be very specific to what environment they function best in1. Numerous environmental impacts were tested for the enzyme peroxidase which catalyzes the decomposition of hydrogen peroxide. The basic decomposition reaction was carried out first without any environmental alterations. The hypothesis for this reaction was supported. The enzyme caused the amount of absorbance increase
Premium Enzyme Chemical reaction Catalysis
Enzymes are generally protein macromolecules that act as catalysts in metabolic reactions. A catalyst is a chemical agent that speeds up a reaction without being consumed by the reaction. Enzymes speed up metabolic reaction rates by lowering the activation energy barrier‚ which is the amount of energy initially needed to spark a reaction. It allows reactant molecules to absorb enough energy to break bonds and react without raising the temperature to an extreme. During this process the substrate
Premium
Affect of enzyme concentration to the rate of reaction Aim: With the experiment of protein solution‚ in this case egg white added to different pepsin concentrations (0%‚ 0.2%‚ 0.4%‚ 0.6%‚ 0.8%‚ 1.0%) shows‚ as the egg white is a protein and the pepsin works as an enzyme‚ how a higher pepsin concentration and therefore a larger amount of enzymes effect the rate of reaction. Hypothesis: An increased concentration of pepsin speeds up the time the mixture needs to come clear. Introduction:
Premium Chemical reaction Chemistry Enzyme
BIO 5 Lab Report: Lactase Enzymes Enzymes are biological catalysts or assistants. Enzymes consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. Enzymes can either launch a reaction or speed it up. The chemicals that are transformed with the help of enzymes are called substrates. In the absence of enzymes‚ these chemicals are called reactants. Enzymes are thought to have an area with a very particular shape. When a molecule of
Premium Enzyme Chemical reaction Catalysis
Temperature and Enzyme Activity Aim: To investigate the effect of temperature on enzyme activity Hypothesis: As the temperature deviates from 40°C the activity will lower Equipment: – Chemicals: – Milk – Junket tablets – Hot water – Ice – Test tubes – Stopwatch – Measuring cylinder Risk Assessment: |Hazard |Risk |Prevention | |Hot Water
Premium Enzyme Chemical reaction Thermodynamics
Anatomy of Flowering Plants Tissues A tissue is a group of cells having a common origin and usually performing a common function. Based on cell’s capability to divide‚ tissues are classified into two main groups which are as follows: 1. Meristematic and 2. Permanent tissues. Meristematic Tissues: Cells in the meristematic tissue are capable of dividing. Meristematic tissues are found in those regions which need to grow continuously. For example‚ root tips and stem tips contain meristematic
Premium Plant anatomy Phloem Plant physiology
Reaction on Reaction Paper The first thing that came to my mind when I have heard about Reaction paper is “What exactly is it?” and before I get an understanding of that‚ I resisted that change. But later‚ when I have studied about it and understood its purpose‚ I have started thinking “Why people avoid change at the first look without an analysis?” The general human tendency is to live in a comfortable zone in which he has lived for quite some time. He draws a circle‚ thinks that circle is his
Free Mind Thought Integer