Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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chemical reaction by decreasing the activation energy. In biology catalyst is called biological catalyst‚ and enzyme is an example of biological catalyst. Enzyme is very important for biochemical reactions‚ as it helps the reaction to become faster than usual. The reactions with enzymes will react 10 billion times faster than those solutions without enzymes. (clitoncc)The enzymes can only react with one particular reaction‚ in order for the reaction to react it needed to find the correct enzyme. (uab)
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ASSIGNMENT in DOMESTIC TOURISM and GEOGRAPHY *Rehabilitation of EDSA* *Reclamation of Manila Bay* Submitted by: NAEG‚ Nelian N. III-CTM Submitted to: Prof. Arnold Germina Reaction Paper Rehabilitation of EDSA: The rehabilitation of EDSA is one of the nicest ideas to ease the traffic in Metro Manila especially it is a major highway and almost everybody use it. In contrast to that‚ Pres. Aquino is right to suspend it and make further study about it. Surely‚ it will take time to
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various household materials for insulation stove or microwave to heat up water Part 1: Heat transfer and human sensation 1. Pour warm water in the bowl on the left‚ cold water in the bowl on the right‚ and room temperature water in the bowl in the middle. Room temperature water should be around 20-22°C. 2. Put your left hand in the warm water bowl and your right hand in the cold water bowl. Leave them in those bowls for one minute. How do your hands feel? _Both hands are burning alittle
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purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity is between 23 to 25 ℃.
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Hypothesis The optimum temperatures of Alcalase and Savinase will be different. Above and below their optimum temperatures activity will decrease. Biological explanation This investigation is designed to look at the effect of temperature on the activity of the proteases Alcalase and Savinase. By the end of it I hope to know the optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin‚ which is a translucent‚ colourless‚ brittle solid
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Engana‚ Mary Gwen Stefani D. IA12117 Aug. 23 2013 Prof. Domingo Barcarse Reaction Paper We all have individual differences‚ as well as Ishaan in the movie that has dyslexia. He is a person that just cannot seem to get anything right in class but are more interested in his surrounding like in arts‚ animals and playing games. Though
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homeostasis to regulate control systems such as body temperature‚ osmotic balance‚ blood pressure‚ blood glucose and respiratory gases in tissues. This report will focus on thermoregulation‚ which is the ability of an organism to keep its body temperature within certain boundaries‚ despite the fluctuating external environment. The purpose of thermoregulation is to balance thermal inputs and thermal losses in the body to maintain a core body temperature of approximately 36.7 degrees celsius‚ despite the
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Biology Enzymes IA Design Introduction: Enzymes are globular proteins‚ they are responsible for most of the chemical activities of a living organism. They act as catalysts‚ substances that affects the reaction of other substances without being destroyed or altered during the process. They are extremely efficient in the body system of living organisms‚ one enzyme may catalyse over a thousand chemical reactions every second. But there are certain conditions that need to be fulfilled in order for the
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