a single line of each culture Bacillus subtilis‚ Pseudomonal aeruginosa‚ and Escherichia coli‚ to each of the separate sections. The plate was incubated until the next lab period‚ where we then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table 1) * In order to test carbohydrate catabolism under anaerobic conditions we tested cultures of Pseudomonas aerugenosa‚ Escherichia coli‚ and Alcaligenes faecalis in tubes containing a glucose medium. After inoculating
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Biology101 McDowall 10/29/2012 Escherichia Coli “The Bacterial Beast” E.coli or binomial name Escherichia coli was discovered by a German pediatrician named Theodore Escherich in 1885. Dr. Escherich originally named the bacteria‚ bacillus communis coli. After the demise of Dr. Escherich the intestinal bacteria was then named Escherichia coli after the late doctor in 1919. The bacteria Escherichia coli are classified as follows. Domain- Bacteria Kingdom- Eubacteria Phylum- Proteobacteria
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The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the transformants were positive for the gene expression. The transformed E. coli on the media appeared fluorescent green under UV light. Introduction The bacteria used in this experiment is Escherichia coli which is not naturally competent.
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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species of Shigella. 1. Shigella sonnei 2. Shigella dysenteriae 3. Shigella flexneri 4. Shigella boydii 4. Colonies that appear red with black centres on XLD are likely to be which of the following organisms:- a. Shigella flexneri b. Escherichia coli c. Salmonella typhimurium
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CHAPTER 4 RESULTS AND DISCUSSION 4.1. Angelika keiskei (Ashitaba )crude non-polar extract The crude non-polar extract from the plant Angelika keiskei (Ashitaba) was tested for the anti-bacterial property by using the Kirby – Bauer Disk Diffusion test. There were four 25uL of Ashitaba non-polar extract with different concentrations used which were 12.5 %‚ 25%‚ 50% and 100%. In line with this‚ respective positive controls of the two specimens and negative control were also used. The results
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combat various physiological and pathological disorders in humans. If the antibacterial protein is isolated and is proved non-cytotoxic to humans‚ then it can be researched and developed further as a drug for curing various diseases caused by Escherichia coli and Staphylococcus aureus ailments in human beings and thus give an impetus to further research on being used as model organisms to be studied. 5.2 DEFINITION OF THE PROBLEM: Treating every bacterial-caused
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters‚ singly Small - Staphylococcus aureus Bacteria 2 Gram negative (pink) Rods (shorter than B. cereus) Rounded‚ regular‚ pair‚ clusters Medium - Escherichia coli Bacteria 3 Gram positive (purple)
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the bacteria grown can ferment lactose. The results acquired by our test was positive with color change to pink. Hence the unknown bacteria was gram-negative and can ferment lactose. Amongst Escherichia coli and Proteus vulgaris‚ the result MacConkey agar confirmed that the unknown bacteria was Escherichia
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