The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Perfume is a mixture of many different products that give the human body‚ animals‚ objects‚ and living species a pleasant scent. "Perfume came from the Latin word "per" meaning "through" and "fumum" or smoke (Dorman‚ page 1)". Many natural and man-made materials have been used on perfume to make the scent usable for skin and clothes. Perfumes does not smell exactly same on different people because of their different body chemistry‚ temperature and body odors. Perfume was first created by the Egyptian
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Title Page: Module Code: FC 511 2T Module Title: Skills for study Tutor Name: Emma Duffy Assessment title: Formative Lab Report: Enthalpy of Combustion of Butanol Student ID Number: 201233920 Date of submission: 6/3/2017 Past tense recapping what you did in the experiment Present tense explain theory and compare theory THINGS TO IMPROVE : Reference Introduction Discussion Avoid Plagiarism Table of Contents Introduction P.3 Aim Materials
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TOXIC) Ethanol (HIGHLY FLAMMABLE and HARMFUL if using Industrial Denatured Alcohol‚ IDA) Propan-1-ol (HIGHLY FLAMMABLE‚ IRRITANT) Butan-1-ol (HARMFUL) One or more other alcohols‚ as available‚ from: Butan-2-ol (IRRITANT) Propan-2-ol (HIGHLY FLAMMABLE‚ IRRITANT) 2-Methylpropan-1-ol (IRRITANT) Sodium carbonate solution‚ 0.5 M‚ about 10 cm3 per ester Method- a Add 10 drops of ethanoic acid (or propanoic acid) to the sulfuric acid in the specimen tube. b Add 10 drops of ethanol (or other
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#15 Synthesis of Ethyl Salicylate from Salicylic Acid ____________________________________________________________ _______________ Purpose: Salicylic acid is converted to the liquid ethyl salicylate by addition of ethanol. Yields of salicylic acid (week 1) and ethyl salicylate will be found‚ as well as the overall yield of the 2-step conversion of acetyl salicylic acid to ethyl salicylate. Introduction: Esters are formed from the reaction of acids and alcohols: O O
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D4 H and E staining procedures Haematoxylin and eosin staining is a technique used by pathologists to add definition to nuclei and other parts of a cell in tissue samples under a microscope so that they can see it better when analysing the cells form and abnormalities. The H&E technique is the most commonly used in histology and the diagnoses of malignancies is based largely on the results of this procedure. The dyes will specifically stain elements of a cell or tissue because the dyes have a high
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Once the ten minutes are up‚ the solids are then isolated by vacuum filtration‚ in a set up that includes a Hirsch funnel‚ and then rinsed with 9.8 mL of hot water and 5.0 m of ice-cold 95% ethanol. Once rinsed‚ the solids were then recrystallized with a 1:3 ratio of ethanol:acetone; which include 10 mL of ethanol and 20 mL of acetone‚ giving us a total volume of 30.0 mL. Once the 1:3 ratio of ethanol:acetone was added‚ the crude produce is then dissolved by heating the solution on hotplate with the
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absolute ethanol (15mls) were combined with three boiling chips in a 50ml round bottom flask. The round bottom flask was placed on a heating plate and was refluxed starting at 109 ̊C. The solution began boiling at 111 ̊C. It was allowed to reflux for approximately 30 minutes and the solid was dissolved. The round bottom flask was then placed in an ice bath and allowed to cool to room temperature. Concentrated 18 M sulfuric acid (2.4mls) was added to the solution. Then absolute ethanol (3mls) was
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Alcohol and it is effects on a human health Alcohol has a long history Humans have drunk alcohol for at least 12‚000 years and it has used in the different religious rituals. For instance the ancient Romans had a god of wine Bacchus and Greeks had also a god of wine Dionysus. Cristian Communion services and certain Jewish religious retuals include wine to the present day. On the other hand wine was usual daily drink in Mediterranean countries and beer was part of the staple diet until the early
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pyruvate splits itself into carbon dioxide and acetaldehyde. NADH is then taken from the acetaldehyde and ethanol is left. (Starr‚ Evers & Starr‚ 2011) Bread making is an example of alcoholic fermentation‚ the yeast being the reason that the bread rising. As explained before the carbon dioxide molecules that are given off during the second stage of fermentation caused the dough to rise and the ethanol that was left bakes out of the bread. Methods and Materials In order to start this experiment
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