Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The
Free Solvent Acetic acid Ethanol
the water from the spinach. Make Ahead 8 cups spinach‚ chopped 16 ounces cottage cheese 8 ounces old cheddar cheese‚ cubed 6 eggs‚ beaten ¼ cup butter‚ softened 2 tablespoons almond flour ½ teaspoon sea salt ½ teaspoon ground nutmeg Butter‚ for greasing the casserole dish 1. Preheat the oven to 350ºF. 2. Lightly grease a 9-by-13-inch casserole dish. Set aside. 3. Place a large saucepan filled with water over high heat and bring the water to a boil. 4. Blanch the spinach until it is tender‚
Premium Cooking Nutrition Food
(Ascorbic Acid)‚ Vitamin D (Cholecalciferol)‚ Vitamin E (Tocopherol) and Vitamin K‚ Calcium‚ Copper‚ Iron‚ Potassium‚ Magnesium‚ Manganese and Zinc. Moringa has 17 times more Calcium than that of milk‚ Moringa‚ with 25 times more Iron than that of Spinach‚ 15 times the potassium in banana assists in the regulation of the acid-base and water balance in the blood and the body tissues. It assists in protein synthesis of amino acids and in carbohydrate metabolism. It has been scientifically proven from
Premium
disks was used during cellular respiration‚ the leaf disks might not have risen even though they did photosynthesize. I think to improve this experiment next time; we have to keep all these factors in mind‚ also make sure to pick the leaves off the spinach plant ourselves‚ or at least ascertain the health and age of both the leaves we use and the plant they are from‚ not to mentioned doing the vacuuming process as well. In general‚ for future experiments‚ I would allot more time and have more materials
Premium Philadelphia Computer software Mobile computing
|Document ID: |Standard Operating Procedures’ Title: |Print Date: | |ORIGIN-CA2 |CASSAVA PROCESSING |08/07/2012 | |Revision: |Written By: |Date Prepared:
Premium
Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
Premium Chromatography Analytical chemistry Silica gel
Fresh spinach leaves | 10g | Fresh solnostemon hybrid leaves | 10g | Chromatography solvent (9:1 petroleum ether-acetone) | 3mL | Measuring cylinder (20mL) | 1 | Strainer | 1 | Acetone (100%) | 40mL | Calcium carbonate | As much as needed | Pestle and mortar | 1 | Electronic balance | 1 | Scissors | 1 | Ruler | 1 | Measuring cylinder (15mL) | 1 | Beaker (50mL) | 1 | Table 2 shows the apparatus and materials used in this experiment METHOD Preparation of spinach pigment
Free Photosynthesis Chlorophyll Thin layer chromatography
spectrum that allows them to process a different wavelength of light for photosynthesis (Wiles‚ 2013). In this experiment we tested varying wavelengths of light to see which would cause photosynthesis to occur to the greatest degree in spinach leaf disks. The spinach leaves were hole-punched to form 60 disks. These disks were placed in a solution of sodium bicarbonate and dish detergent to create a vacuum and remove all of the oxygen from their environment. This vacuum caused the disks to sink‚ and
Premium Photosynthesis Electromagnetic radiation Wavelength
Cheese and Garlic Naan Spinach & Garlic Naan Spinach & Cheese Naan Spinach‚ Cheese & Garlic Naan Assorted Naan & Dips 9.95 Chef’s selection 3.90 ACCOMPANIMENTS R a i t a – A cucumber & yoghurt salad. The perfect match for hot curries! Mango Chutney‚ Lime‚ Mixed or C h i l l i p i c k l e Homemade & delicious! Pappadums Mild Medium Rice : $2.50 per person One Bill per Table Hot APPETISERS P a k o r a s ( B h a j i ) ( 4 ) – Onion‚ spinach & chickpea fritters
Premium Indian cuisine Fruit
Many nutrients‚ including the ones that fight against cancer and heart disease are found in fruits and vegetables. 4. Vegetables have less sugars than fruits. Spinach 5. Spinach is an excellent example of a nutrient-dense vegetable. Spinach provides at least 50 % of the RDI for vitamin A‚ and about 20% of the RDI for vitamin C. Spinach is a good source of iron‚ calcium‚ and folate. Asparagus 6. Asparagus is high in folate content (20 to 50% of the RDI per serving). Folate is a nutrient identified
Free Nutrition Vegetable