higher temperatures. 3. State how sucrase activity changes with increasing sucrose concentration. Discussion 1. Explain how pH and temperature affect enzyme activity. 2. Compare optimal temperature for sucrase activity to body temperature. 3. Specifically state where in the intestine sucrase is likely to be most active (pH along GI tract). 4. Explain how sucrase activity in the intestine is affected by the sucrose content of food. Why is this important? 5 Heat treatment was used to denature
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Glomerular Filtration Activity 1: Effect of Arteriole Diameter on Glomerular Filtration 1. Compare this data with your baseline data. How did increasing the afferent arteriole radius affect glomerular filtration rate? >>Increasing the afferent arteriole radius pushed the glomerular pressure‚ the glomerular filtration rate‚ and the volume of urine to be higher than the baseline data. Increasing the afferent arteriole radius increased the glomerular filtration rate. 2. Under
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AP Biology Unit Test Review Guide Plants Obtaining and Transporting Nutrients Pigments and photosynthesis 1. Distinguish between the strategies used by autotrophs and heterotrophs to obtain free energy for cellular processes. In other words‚ how and in what form does each capture and store that energy? 2. Explain how photosynthesis differs in eukaryotic organisms (with organelles such as chloroplasts) and in prokaryotic organisms (single-celled‚ no organelles). 3. How does a metabolic
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Biology 100 Lab Laboratory Report Title: Identifying the four major classes of macromolecules through various tests. Group Members: Antonia Johnson‚ Payton Ewing‚ Lenneisha Hepburn‚ Kendisha Hanna‚ Introduction: Macromolecules also known as biological molecules are monomers which are the simple units of polymers. All macromolecules contain carbon and hydrogen which are found in organic compounds. In this Experiment we will test for the presence of protein‚ starch‚ lipids‚ and sugars by analyzing
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Introduction A series of biochemical tests was carried out to identify unknown carbohydrates. There were six carbohydrates that needed to be identified; they have been randomly labelled A-F. The carbohydrates are glucose‚ fructose‚ maltose‚ lactose‚ sucrose and starch. There was six tests that were carried out to help identify them‚ these were: Iodine Test‚ Solubility in Water‚ Benedict’s test‚ Acid Hydrolysis‚ Barfoed test and Diastix test. Aim The aim of the experiment which was carried out was
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Firstly‚ there was a source of error when testing sucrose for a carbohydrate presence using Benedict’s Regent. According to Dr. Hunt‚ the sucrose solution should have remained blue but instead the sucrose turned teal in colour. This could be a result of mixing different solutions by accident. Fortunately‚ this was a very minor issue which was unfortunately over looked. The next
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most fruits come from its sugar content and these sugars that the fruits contain are known as invert sugars. In this experiment‚ an orange was used. An orange which is an excellent source of vitamin C gets its sweetness from natural sugars which are sucrose‚ glucose and fructose (livestrong.com). In this experiment the concentration of glucose in an orange was measured. There are various methods which can be used to measure the glucose concentration but in this experiment a highly specific enzymatic
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of this experiment is to investigate the osmosis effect in potato cores while they are submerged in different concentrations of sucrose solution. Osmosis will take place while the potatoes are fully submerged in the sucrose solution. Apparatus æ Scalpel (1) æ Tile (1) æ Pr. Tweezers (1) æ 250ml Beakers (2) æ 100ml Water (Per Beaker) æ 100ml Sucrose Solution o 0.2M o 0.4M o 0.6M o 0.8M o 1.0M æ Measuring Cylinders (2) æ Ruler (1) æ Potato Cores (30) æ Stop
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one gains the distilled water. MATERIAL LIST 2-250ml beakers. 25ml distilled water 25ml sucrose solution(sugar) 2 strings. 2 dialysis tubing. PROCEDURE. Obtain 2 strips of 15cm dialysis tubing. Tie a knot n one end of each piece of dialysis tubing to form 2 bags. Pour 25ml of each of the following solutions into separate bags: a. Distilled water. b. Sucrose(sugar). remove most of the air from each bag by drawing the dialysis bag between two fingers. Tie
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Gel Filtration Gel filtration is a non-adsorptive chromatography technique that separates molecules on the basis of molecular size. Desalting and buffer exchange are two special examples of gel filtration that are widely used in many downstream bioprocesses. Desalting is used to completely remove or lower the concentration of salt or other low molecular weight components in the sample while buffer exchange replaces the sample buffer with a new buffer. Gel filtration is one of the easiest chromatography
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