gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the transformants were positive for the gene expression. The transformed E. coli on the media appeared fluorescent green under UV light. Introduction The bacteria used in this experiment is Escherichia coli which is not naturally competent. E. coli is a gram negative rod shaped bacteria and a facultative anaerobe. This bacteria forms part of the bacterial
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CHAPTER I INTRODUCTION Background of the study For most indigenous students living in remote areas access to secondary schooling‚ especially to the upper levels‚ means leaving home to go to a school which makes provision for boarding. Some of the schools providing these sorts of opportunities have been doing so for more than 50 years‚ with well established links to particular communities and families. However the increase in the number of young people being born and growing up in remote communities
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Cars Instead of selling your car or sending it to the junk yard‚ donate it to a charitable organization. Not only will you keep the car out of the landfill‚ you will receive a tax deduction as well. Clothes Clothes are one of the easiest things to recycle as so many charitable organizations will accept them such as Goodwill‚ Veteran’s Support Groups and most churches. In addition‚ you can possibly sell them at a consignment shop if they are still in good shape and in style. Christmas Trees
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research. There are many types of microscopes available including acoustic or ultrasonic‚ compound‚ fluorescent or ultraviolet (UV)‚ inverted‚ laser or confocal‚ polarizing‚ portable field‚ scanning electron microscope (SEM)‚ scanning force or atomic probe microscope (SFM/AFM)‚ stereoscopes and transmission electron microscopes. Acoustic and ultrasonic microscopes‚ compound microscopes‚ and fluorescent and UV microscopes are available. Acoustic and ultrasonic microscopes use sound waves to create images
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Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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1. ELEMENTS PICTURE (http://images-of-elements.com/xenon-2.jpg) 2. DESCRIPTION OF XENON The name originates from the Greek word ’xenon’ meaning the stranger. (http://www.elementalmatter.info/element-xenon.htm) Xenon is the fifth member of the noble gases‚ and makes up one part of a twenty millionth of the atmosphere. It is colorless‚ tasteless‚ odorless‚ and a non metal element. When put in a vacuum tube‚ it creates a bright blue glow because of the electrical discharge. Xenon
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The ABC’s of Nondestructive Weld Examination An understanding of the benefits and drawbacks of each form of nondestructive examination can help you choose the best method for your application BY CHARLES HAYES * Acknowledgement: The Paper was first published in the Welding Journal May 1997 published by the The American Welding Society‚ 550 NW LeJeune Road‚ Miami‚ FL 33126. | CHARLES HAYES is International Sales/Support Manager‚ The Lincoln Electric Co.‚ Cleveland‚ Ohio. He holds NDT Level
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Why do computer screens etc use red‚ GREEN & blue? February 16‚ 2005 12:50 PM Subscribe The primary colours are red‚yellow & blue. All other colours can be made from them. Why then do computer screens etc use red‚ GREEN & blue? posted by TiredStarling to computers & internet (23 answers total) 1 user marked this as a favorite Quoth the Wiki: "The human eye contains photoreceptor cells called cones which normally respond most to yellowish-green‚ green‚ and blue light (wavelengths of 564nm
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DNA QUESTION 1995: L. PETERSON/AP BIOLOGY The diagram below shows a segment of DNA with a total length of 4‚900 base pairs. The arrows indicate reaction sites for two restriction enzymes (enzyme X and enzyme Y). (A) Explain how the principles of gel electrophoresis allow for the separation of DNA fragments. (B) Describe the results you would expect from electrophoretic separation of fragments from the following treatments of the DNA segment above. Assume that the digestion
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GREEN MARKETING PRACTICES OF INDIAN FIRMS Introduction According to the American Marketing Association‚ green marketing is the marketing of products that are presumed to be environmentally safe. Thus green marketing incorporates a broad range of activities‚ including product modification‚ changes to the production process‚ packaging changes‚ as well as modifying advertising. Yet defining green marketing is not a simple task where several meanings intersect and contradict each other; an example of
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