to buy their meal. In peak hours‚ there are at least 20 people in the queue. In off peak hours‚ there are still 10 people in the queue. A survey interviewed 10 customers who standing outside the Pepper Lunch restaurant‚ asking whether they would give up if they saw a long queue outside the restaurant. Not surprisingly‚ 5 people said yes and they would choose other restaurant. The result showed that long queuing time is a serious problem to pepper lunch restaurant. Base on the above problems‚ we
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transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both pSA11 and pDH223
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How to Write a Sonnet Learn to write a sonnet in iambic pentameter‚ just like Shakespeare did. Discover the rhythm and rhyme scheme of the quatrains and couplets that make up a Shakespearean sonnet. Here are the rules: * It must consist of 14 lines. * It must be written in iambic pentameter (duh-DUH-duh-DUH-duh-DUH-duh-DUH-duh-DUH). * It must be written in one of various standard rhyme schemes. If you’re writing the most familiar kind of sonnet‚ the Shakespearean‚ the rhyme scheme
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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acceptance‚ now try to relate the law on the mode of acceptance with the fact of the case in the question by checking whether there is an acceptance or not? 4. CONCLUSION Now‚ it is a time to conclude your answer. You may sum up what you have discussed earlier by giving simple conclusion like “As a conclusion‚ in order to form a valid contract‚ the offeree must communicate his acceptance to the offeror. Silence by the offeree is not a valid acceptance and therefore there will
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different products. Close up is targeted at young people; the target market being “Multi Brand Households” where the young does not use what their parents use. This particular targeting was significant when Close Up was launched because Colgate positioning was a sort of oral care and hygiene benefits .Also‚ Colgate was going for a broad market constituting of all the age groups. Positioning A large part of Close Up’s success is because of its brand positioning. " Close up falls under the third
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1. The overall goal of this lab was to carry out experiments that clearly demonstrate the different ways DNA genetic information‚ specifically transduction and conjugation. The first half of this experiment focused on exploring the mechanisms of transduction. This was done by creating a spot titer plate for phage carrying kanamycin resistance and E. coli. E. coli was then proven to have gained kanamycin resistance throughout transduction as demonstrated by its ability to grow on a medium containing
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East West University Department of Economics Course Outline (Fall 2008) MDS 501: ECONOMICS OF DEVELOPMENT Instructor: Muhammad Sirajul Haque‚ Ph.D Professor and Chairperson‚ Department of Economics Office Hours: Friday : 04:40 P.M to 06:00 P.M Friday 06.00 P.M to 07:30 PM Class Hours: F - 3:00 – 6:00 P.M.; Room # ADMN-1318 Brief Description: Underdevelopment which is a major problem in the developing world is the central focus of this course. It examines the nature and
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General Guidelines Essays should be a minimum of 500 words‚ typed. This length requirement is to give you a sense of the minimum level of detail expected in your writing. Essays should demonstrate that you understand the information well enough to explain your ideas in your own words and can give examples where appropriate. Plan to use course resources to complete the essays and to cite your sources using the MLA Format. In fact‚ as this is an essay exam‚ you should not use outside sources for
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3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
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