Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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FOOD NUTRITION BASICS LABORATORY REPORT LAB 3 PROTEINS DUE DATE Next FNB Practical NAME (CAPITALS) ______________________________GROUP LETTER___________ This work is the product of my own efforts and has not been copied from any other sources except where full acknowledgement has been given. Signed________________________________________ Student No._____________ Introduction The principle involved in this experiment is denaturation. It involves loss of ordered structure and can be caused by changes
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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CHEMISTRY LAB REPORTS How to Write a Lab Report Lab Reports gives the explanation of the experiment done Lab reports are an essential part of all laboratory courses and usually a significant part of your grade. If your instructor gives you an outline for how to write a lab report‚ use that. Here ’s a format for a lab report you can use if you aren ’t sure what to write or need an explanation of what to include in the different parts of the report. A lab report is how you explain what you did
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Niharika Palakodety‚ Victoria Wang‚ and Grace Xiong Mrs. Rhonda Smith Honors Chemistry Period 4 13 May 2012 Redox Reactions Lab Report We wished to investigate the oxidation number of a metal that would form aqueous ions when reacted with an aqueous nitrate salt. For this purpose‚ we chose to investigate the reaction of solid copper metal‚ in the form of a wire‚ with aqueous silver nitrate. Before we began the lab‚ we hypothesized that when silver nitrate‚ AgNO3‚ reacts with copper‚ Cu‚ the
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IB Chemistry Experiment 4 Composition of a Post-1983 Penny Lab Report Background: Through the use of percent composition one can easily find out how much of a certain element is in a compound or an item such as a post-1983 penny. The experiment will be using HCl a strong acid to dissolve all the zinc inside the outer copper shell. The reaction follows as such; Zn+2HCLZnCl2+H2‚ but there will be no reaction with copper. The portion that will be measured is the mass of copper left after all of
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15g of chicken liver for 1% catalyzes concentration using electronic balance and a sterile weigh boat Grind the chicken liver until little to no clumps are visible Measure 15 ml of distilled water using graduated cylinder and transfer to mortar and pestle containing the chicken liver Using the sterile transfer pipet‚ transfer 1ml of hydrogen peroxide to each test tube Transfer 5ml of catalase concentration mixture to a different test tube Using a ruler‚ align the test-tube parallel to it Start
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate that responds with an enzyme is used to
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for pH values above 7‚ each of which is ten times more alkaline than the next lower whole value. For example‚ pH 10 is ten times more alkaline than pH 9 and 100 times (10 times 10) more alkaline than pH 8. Primary pH standard values are determined using a concentration cell with transference. This measures the potential difference between a hydrogen electrode and a standard electrode. Measurement of pH for solutions can be done with a glass electrode and a pH
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