Molecular Biology Lab Report Payton Jackson Introduction In this lab‚ I am going to use antibiotic-resistance plasmids to transform Escherichia coli. Materials For this lab you will need the following: LB Agar Petri dishes Beakers Test tubes CaCl2 solution Sensitive E. coli (-ampR) amp plasmids ampicillin -amp cells Water bath to heat shock cells A freezer to incubate cells Process Step 1: Wash hands and sanitize lab setting. This will prevent anything reacting with a
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Morgan Kimmerle Monday Lab Dr. McCorkell Abstract The purpose of this study was to cross and analyze the reciprocal crosses of wild flies and mutant flies. In this lab Drosophila melanogaster commonly known as fruit flies were used to understand some important genetic principles that were once proposed by Gregor Mendel. Mutant traits can be autosomal dominant‚ autosomal recessive traits‚ or even sex-linked dominant and sex-linked recessive. F1 and F2 generations were obtained by performing
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The underlining purpose of the experiment was to show that error is ok as long as you know what the flaws are and be able to explain they happened. The lab involved burning a Marshmallow‚ a CHEEZ-IT‚ a Cheeto‚ and A Chex. The heat from the burning snack food is then carried into the H2O and used to calculate the energy content. An improvement that could be made was a replacement for the paperclip; it was difficult to balance the paperclip
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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The purpose of this lab is to successfully infiltrate E. coli bacterial cells with a pARA-R plasmid that is antibiotic resistant and has the rfp gene‚ or red fluorescent protein. This can be verified if the E. coli obtains the characteristics of the plasmid when it enters. To start‚ three Petri plates containing agar are needed. On each plate there is a control group and a treatment group; the treatment group being the one with the plasmid. Before the plasmid is put with the E. coli‚ first the bacteria
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Aspirin were used to relieve pain‚ inflammation‚ and fever. Aspirin were made using a combination of salicylic acid and acetic anhydride.The goal of this experiment was to determined the purity of aspirin using different methods. These methods included‚ reacting salicylic acid with FeCl3‚ finding its melting points‚ and by doing Thin-layer chromatography. When you reacted salicylic acid with FeCl3‚ changes in color will occur. Pure Aspirin has a melting point around 135°C and a pure salicylic acid
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Abstract: Two parts of this lab were performed involving photosynthesis and cellular respiration. The first part of the lab consisted of cutting out spinach leaf disks with a straw and then putting them into syringes containing an infiltration solution and sodium bicarbonate. Then the syringes were place under the presence of light and watched as certain disks floated. This part of the lab consisted of watching photosynthesis take place. Then for the second part of the lab we tested cellular respiration
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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Signature ……………………………….. Date ……………………………….. Total Word Count 1492 I declare that‚ except where specifically indicated‚ all the work presented in this report is my own and I am the sole author of all parts. Toxic shock syndrome (TSS) is an uncommon but serious systemic infection which can occur in males and females of all ages. Symptoms include fever‚ hypotension and desquamation of the skin. The two main micro-organisms that cause
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Introduction In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and
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