against the bacteria by measuring zone of inhibition. Inhibiting concentrations used for all the extracts was 500 mg/ml. Methanolic extract of C. album was found to be most active among all other extracts and inhibits Staphylococcus epidermidis‚ Bacillus subtilis‚ Staphylococcus aureus‚ Escherichia coli. The extract of a mixture of solvents was also found to be effective in inhibiting Staphylococcus aureus. Methanolic extract of C. album was found to give a most clear zone of inhibition. The antibacterial
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(living can only arise from preexisting living) Pasteur ¡V air contained‚ but did not produce‚ microbes (broth‚ s-flasks); pasteurization; lose virility‚ still immunity Lister ¡V aseptic surgery (phenol on surgical wounds) Koch ¡V cause of anthrax (Bacillus anthracis) ¡V bacteria in blood‚ injected into healthy‚ died‚ compared bacteria Jenner ¡V vaccines (cowpox provided immunity against small pox) ¡V milk maids Erlich ¡V ¡§magic bullet¡¨; salvarsan against syphilis Fleming ¡V Penicillium chrysogenum
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sludges‚ sediments etc. Disadvantages of Petri film include: There is no visual difference between the different kinds of bacteria growing on the Petri film and you can’t do any testing on the colonies. No classifying as bacilli or cocci‚ no gram-staining‚ no
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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Shigella Introduction: Shigella is a very infectious bacteria that causes Shigellosis. I. Morphology of Shigella • Gram-negative • Nonmotile bacillus II. 4 different species of Shigella • S. dysenteriae o Most severe type of shigellosis because it secretes Shiga Toxin Shiga toxin- stops protein synthesis in host’s cell. • S. flexneri o Is the predominate species in developing countries. • S. boydii • S. sonnei o Is the most common species isolated in industrialized nations.
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|antibiotics.| ____ 5. Where are you likely to find a photoautotroph? a.|in your refrigerator| b.|in the darkness of the ocean| c.|in your digestive system| d.|near the surfaces of lakes‚ streams‚ and oceans| ____ 6. A method called Gram staining is used to tell a.|what shape a prokaryote has.| b.|how a prokaryote obtains energy.| c.|what kind of cell wall a prokaryote has.| d.|whether a prokaryote has flagella.| ____ 7. Which of the following describes a role of bacteria in the
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from CO2 Synthesizes organic carbon molecules using inorganic carbon. (CO2) Example: Plant; Performs photosynthesis. Other Example: Sulphur bacteria: bacteria may use other organic compounds (oxidation reactions‚ etc.) Bacillus bacteria: Bacteria which is rod-shaped‚ gram-positive‚ aerobic and anaerobic. Found in soil and water. Useful in antibiotics‚ as they can serve to protest the host from
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in Egypt. A German physician and bacteriologist‚ Robert Koch traveled with a group of German colleagues from Berlin to Alexandria‚ Egypt in August‚ 1883. Following necropsies‚ they found a bacillus in the intestinal mucosa in persons who died of cholera‚ but not of other diseases. He reasoned that the bacillus was related to the cholera process‚ but was not sure if it was causal or consequential. He stipulated that the time sequence could only be resolved by isolating the organism‚ growing it in
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Robert Hooke‚ reported to the world that life’s smallest structural units were "Little boxes" or "Cells" as he called them. This discovery marked the beginning of Cell theory. Though Hooke’s microscopes were capable of showing cells‚ he lacked the staining techniques that would have allowed him to see the microbes clearly. The Dutch merchant‚ Anton Van Leeuwenhoek‚ was probably the first to actually observe living microbes through magnifying glasses. Between 1673 and 1723‚ he wrote a series of letters
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aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the methyl red test indicated that no mixed acid fermentation occurred. The DNase test was performed and
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