This section analyzes and presents the results of the two trials conducted‚ including the trial in which Hydrogen peroxide(H2O2) was denatured by a cold catalase solution and the other in which the Hydrogen peroxide(H2O2) was denatured by a hot catalase solution. The experiment conducted displayed signs of experimental error as there were many situations in which the materials provided could have faulted. Theoretically speaking‚ from the inconsistent values provided for the pH levels‚ a possible
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Introduction Catalase is an enzyme that promotes the decomposition of hydrogen peroxide to water and oxygen gas: H2O2 H2O + O2. Hydrogen peroxide is a potential hazard for the body‚ which is why it is used to kill bacteria (WKU‚ 2010). Cells would die if hydrogen peroxide is not broken down by catalase. The activation energy for the decomposition of hydrogen peroxide is reduced from 72 to 20kcal/mol with the presence of catalase. Catalase is specifically
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enhancers added to the luminol. The substances to be compared in this study are as follows: cysteine‚ Cu (II)‚ silver nanoparticles (AgNPs)‚ and zinc sulfide nanoparticles (ZnS-NPs). The first experiment focused on the reaction between alkaline luminol H2O2‚ the most common luminol solution‚ and cysteine‚ one of the twenty essential amino acids. In this experiment‚ 105 g=mL cysteine solution‚ 2.0 105 mol=L Cu(II) solution‚ and the mixture of 1.0 105 g=mL cysteine with 2.0 105 mol=L Cu(II) were tested
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even be damaged‚ such as when exposed to too much heat. A damaged enzyme may no longer work to catalyse a chemical reaction. (Science Buddies on March 8‚ 2012) Aim : The objective of these experiments is to investigate the change in the decomposition rate between the catalase and hydro peroxide with cooked and raw potato and liver. Hypothesis: With consideration to previous studies done into the topic. It is hypothesized that the raw food will provide a higher rate of reaction than the cooked
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Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in the other assays. These variables included a boiled‚ frozen and then thawed‚ and frozen potato extract and dH2O instead of the potato extract. It was noted that the temperature
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the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of O2 production. 3) Discard the used slide container. Results: Organisms Bubbles formation Catalase Patient
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Biology investigation: Liver and hydrogen peroxide By Cuong Tran Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced‚ while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found
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Enzymes are organic catalysts; where a substance speeds up the rate of chemical reactions without changing being changed by the reactions. In lab they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area)‚ temperature on function‚ reusing the catalase‚ reaction rate of iced liver returned to room temperature‚ and effects of pH on enzyme activity‚ to see how much O2 was released
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Name: Charity Flosser Date: 12.17.2012 Unit: Lesson: Unit 4 Lesson 11 Classifying Reactions Objectives: Identify the four most common types of reactions: synthesis‚ decomposition‚ single replacement‚ double replacement Predict the products of reactions Balance chemical equations Part I: Reaction between zinc and iodine Watch the Video. http://www.youtube.com/watch?v=xT9V7Y1iKYc Observations: Describe what happened when the water was added. A reaction
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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