are used to break down these chemicals. The name of the enzyme that was the main focus of the lab is catalase. Catalase is responsible for catalyzing hydrogen peroxide into oxygen and water. Since hydrogen peroxide is continuously produced by numerous metabolic reactions‚ catalase helps to prevent the body tissues from being damaged by the peroxide. pH is used to measure the acidity or basicity of a solution and is very important when chemical reactions are concerned. A value of 7 is neutral while
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nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of substrate on the enzyme’s reaction. Research question: What does the changes in the amount of substrate on an enzyme’s reaction effect on? Hypothesis: I forecast that the more concentrated the hydrogen peroxide is the higher the volume of the foam is and the less concentrated the hydrogen peroxide is the lower the volume of the
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Determining the Effect of Varying Substrate Concentration Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)? Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide‚ into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered‚ can alter the procedure of
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Table of Contents: CHAPTER I: Introduction . . . . . . . . . . . . . . . . . . . . . . .i Statement of the problem . . . . . . . . . . . ii Hypothesis . . . . . . . . . . . . . . . . . . . . . . . . iii Scope and limitation . . . . . . . . . . . . . . . iv Definition of terms . . . . . . . . . . . . . . . . . v CHAPTER II:RRL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi CHAPTER III:Methodology . . . . . . . . . . . . . . . . . . . . . . vii CHAPTER IV:Results
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as the potato‚ hydrogen peroxide is broken down to release water and oxygen. Mixtures were allotted time to react‚ with those have a longer time resulting in more substrate being able to be acted upon in correlation. The sulfuric acid was added after the allotted time and acted as an inhibitor. The inhibitor left a mixture of the products and original hydrogen peroxide. Our titrant of potassium permanganate (KMNO4) shows through its purple color the remnants of the hydrogen peroxide‚ which we were
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mortar & pestle‚ 4 test tubes‚ test tube rack‚ spatula‚ hydrogen peroxide 6%‚ 3%‚ 1.5%‚ pipettes‚ scalpel‚ ruler. Procedure 1. Cut using scalpel several raw potato cubes each measuring 1 cubic centimeter (cc). 2. Each cube was ground with a mortar & pestle and then added to a test tube using a spatula. 3. Test tube 1 was left as the control with no added hydrogen peroxide. 4. Different concentrations of hydrogen peroxide solution were added to each test tube using a pipette. (5mL
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Chemiluminescence of Luminol Introduction In this experiment‚ luminol was mixed with hydrogen peroxide (an oxidising agent) resulting in chemiluminescence. The experimentis designed to see how the duration of luminescence is affected by varying temperatures. It has been made to be deliberately inaccurate‚ so another objective would be to see whether subjective observations can be improved by repetition and averaging. Chemiluminescence is the production of light from a release of energy in
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substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it rise to the surface of the hydrogen peroxide. The time it takes for the filter to rise is an indication of the rate of the rate of enzyme activity. Rate of enzyme activity-distance time Materials: 1. A specific weight enzyme 2. Filter papers 3. 3% Hydrogen Peroxide solution
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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Effect of Substrate Concentration on Catalase Aim: An experiment to find out how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information: Enzymes such as catalase are globular protein molecules with catalytic properties. A catalyst is a substrate which can alter the rate of reaction without itself undergoing any permanent change. As they are not changed
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Practical Report: Background Information: Hydrogen peroxide (H2O2) is a combination of hydrogen and oxygen. In high concentrations‚ it can be unstable and even poisonous. In lower concentrations‚ such as the types found in many homes‚ it works well as a disinfectant and antiseptic.The reason behind foaming of Hydrogen peroxide is because blood and cells contain an enzyme called catalase. Since a cut or scrape contains both blood and damaged cells‚ there is lots of catalase floating around.When
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