produces hydrogen peroxide‚ which is extremely poisonous. When the catalase comes in contact with the hydrogen peroxide‚ it is broken down into water and oxygen. Aim: The aim is to investigate and determine the effect of change in pH on the activity of the enzymes catalase. Equipment: Test tubes Test tube rack Pipettes Stopwatch Ruler Pen Safety goggles Gloves Materials: Liver pureed 3% of hydrogen peroxide (H2O2) with few drops of detergent Safety: Hydrogen peroxide
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enzyme-catalyzed reactions‚ we made fifteen tubes that contained reaction buffer‚ hydrogen peroxide‚ turnip extract‚ and the dye. These reagents were placed in large bottles and were labeled with a sharpie. We gathered fifteen small test tubes for testing and three large test tubes to fill it with stock solutions needed to carry out the experiment. The large test tubes were filled with buffer‚ dye‚ and hydrogen peroxide. Each test tube made contrasted in the amounts of solutions used. The odd numbered
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molecules enclosed in the enzyme Catalase: found in every living thing Takes two molecules of hydrogen peroxide and converts it irreversibly to create oxygen gas and water 2H2O2O2+2H2O Question: What variable affects the rate of enzyme catalysis most? Variables Tested: Hydrogen Peroxide concentration‚ yeast concentration‚ heat and pH Materials: 10% glucose mixture 1.5 %‚ 3% and 6% peroxide mixture Yeast Petri dishes Graduated cylinders Glass Beakers (size varies) pH 3.5 solution
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An investigation to see how much oxygen is given off when different concentrations of catalase are added with hydrogen peroxide. Aim: To see if changing the concentration of catalase (found in celery) with hydrogen peroxide affects the amount of oxygen given of. Background Information: (Hydrogen peroxide - H2O2 1/2O2+H2O) Enzymes: Hundreds of chemical reactions happen simultaneously inside living cells and it’s the job of enzymes to control and regulate the various metabolic
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organisms. The rate of enzyme activity is influenced by temperature‚ pH‚ and the presence of inhibitors. Catalase is an enzyme which is produced by every cell to break down hydrogen peroxide. Hydrogen peroxide is a waste product of cellular activity that is poisonous to cells. Catalase speeds up the decomposition of hydrogen peroxide into harmless water and oxygen gas. The purpose of this lab was to determine whether an inhibitor affects the rate of activity for the enzyme catalase. Since catalase
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FHSB1214 Unit description Biology I Year and trimester of study 2015‚ Trimester 1 Title of lab report Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition. Lecturer’s name Norkhalidah Binti Jamali Investigation of the Enzymatic Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Comments are indicated by ‘>>>’ (in detail)‚ ‘???’ (unclear) or ‘xxx’ (wrong concept) FHSB1214 Biology I (201505)-Lab Report Marking Scheme (Practical
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is the effect of various temperatures‚ 0°C‚ room temperature‚ 37°C‚ 50°C‚ 60°C‚ on the number of oxygen gas bubbles liberated‚ in a decomposition reaction between the enzyme Catalase‚ obtained from crushed mung beans‚ and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins that have a 3-Dimesional shape and contain a region called the
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11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity studies reflect
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Concentration of Hydrogen Peroxide on the change in reaction rate of liver catalase. Hypothesis: Null Hypothesis: If the concentration of the Hydrogen Peroxide is changed then there would be no change in the reaction rate. Alternate Hypothesis 1: I there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will increase. Alternate Hypothesis 2: If there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction
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pipette • Ice bath • 0.1% hydrogen peroxide solution • 3 thermometers • Liver puree • Warm-water bath • 25-mL graduated cylinder • Clock or watch with second hand • 5 50-mL beakers • Paper towels • Filter-paper disks Part A: Observe the Catalase Reaction 1. Put on apron and safety goggles. Use forceps to place a small piece of raw liver in an open petri dish. Use a dropper pipette to put a drop of hydrogen peroxide solution on the liver. CAUTION: Hydrogen peroxide can be irritating to skin
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