Concentration Of Catalase Essay
An investigation to see how much oxygen is given off when different concentrations of catalase are added with hydrogen peroxide.
Aim: To see if changing the concentration of catalase (found in celery) with hydrogen peroxide affects the amount of oxygen given of.
Background Information: (Hydrogen peroxide - H2O2 1/2O2+H2O) Enzymes:
Hundreds of chemical reactions happen simultaneously inside living cells and it's the job of enzymes to control and regulate the various metabolic activities. Enzymes are biochemical catalysts, which speed up reactions that would otherwise be too slow to be of any use to an organism. They control cell metabolism by regulating how and when reactions occur. Enzymes are globular proteins; they …show more content…
Gas Syringe 1 It is easily read, set up and is scientific. I will use this instead of downward displacement because downward displacement can let oxygen slip out of the test tube, decreasing the amount of oxygen recorded.
Test Tubes 6 To enter all substances in and easy to get hold of.
Measuring Beaker 3 To enter all liquids in to keep them separate, and to measure them out before mixing them.
2.5 ml Syringe 3 To inject the liquids into the test tube and to get the correct measurements.
Stop Watch 1 To measure the time so I can stop the experiment after 2 minutes.
Thermometer 1 To check the room has a constant temperature whilst doing the experiment.
Universal Indicator Paper 1 pack To make sure the pH level doesn't change.
Clamp 2 To hold the gas syringe up.
Hydrogen Peroxide 15ml To mix with the catalase
Catalase 10ml To mix with the hydrogen peroxide
Water 10ml To dilute the catalase.
Method:
In this investigation I am going to use 6 different concentration of catalase. (100%, 80%, 60%, 40%, 20% and 0%) I am also going to repeat the experiment 3 times so I can take the average and gain more reliable …show more content…
I used the gas syringe because it was the most precise piece of equipment that I could use that was available to myself.
The experiment was suitable because it tested exactly what I wanted it to do.
The experiment had no anomalous results; everything fitted the trends of the experiment clearly. My results are reliable because in each three of the replicas I carried out, all the numbers were similar and didn't differ by great deals from one another.
Out of all the errors the ones that caused the most problems were the time lapses because this will definitely affect my results. More oxygen would have been allowed into the gas syringe than what I was measuring for and the oxygen was allowed to enter which could have been the fastest part of the whole reaction and allowing it to escape. And the time lapse between the hydrogen peroxide entering and the bung being put in. this again was probably the fastest part of the reaction which I missed by putting the bung in to late.
Even with the time lapse and the delay using the gas syringe I would still say my results were reliable, there was no anomalous results, and all my results were around measured around the same level of oxygen given
Aim: To see if changing the concentration of catalase (found in celery) with hydrogen peroxide affects the amount of oxygen given of.
Background Information: (Hydrogen peroxide - H2O2 1/2O2+H2O) Enzymes:
Hundreds of chemical reactions happen simultaneously inside living cells and it's the job of enzymes to control and regulate the various metabolic activities. Enzymes are biochemical catalysts, which speed up reactions that would otherwise be too slow to be of any use to an organism. They control cell metabolism by regulating how and when reactions occur. Enzymes are globular proteins; they …show more content…
Gas Syringe 1 It is easily read, set up and is scientific. I will use this instead of downward displacement because downward displacement can let oxygen slip out of the test tube, decreasing the amount of oxygen recorded.
Test Tubes 6 To enter all substances in and easy to get hold of.
Measuring Beaker 3 To enter all liquids in to keep them separate, and to measure them out before mixing them.
2.5 ml Syringe 3 To inject the liquids into the test tube and to get the correct measurements.
Stop Watch 1 To measure the time so I can stop the experiment after 2 minutes.
Thermometer 1 To check the room has a constant temperature whilst doing the experiment.
Universal Indicator Paper 1 pack To make sure the pH level doesn't change.
Clamp 2 To hold the gas syringe up.
Hydrogen Peroxide 15ml To mix with the catalase
Catalase 10ml To mix with the hydrogen peroxide
Water 10ml To dilute the catalase.
Method:
In this investigation I am going to use 6 different concentration of catalase. (100%, 80%, 60%, 40%, 20% and 0%) I am also going to repeat the experiment 3 times so I can take the average and gain more reliable …show more content…
I used the gas syringe because it was the most precise piece of equipment that I could use that was available to myself.
The experiment was suitable because it tested exactly what I wanted it to do.
The experiment had no anomalous results; everything fitted the trends of the experiment clearly. My results are reliable because in each three of the replicas I carried out, all the numbers were similar and didn't differ by great deals from one another.
Out of all the errors the ones that caused the most problems were the time lapses because this will definitely affect my results. More oxygen would have been allowed into the gas syringe than what I was measuring for and the oxygen was allowed to enter which could have been the fastest part of the whole reaction and allowing it to escape. And the time lapse between the hydrogen peroxide entering and the bung being put in. this again was probably the fastest part of the reaction which I missed by putting the bung in to late.
Even with the time lapse and the delay using the gas syringe I would still say my results were reliable, there was no anomalous results, and all my results were around measured around the same level of oxygen given