Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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In this article titled Suspects in Video Beating Could Get Life in Prison by Rich Phillips from CNN is about eight teens beating and attacking another teen girl violently for “spreading rumors” on the internet and trying them as adults in court. The eight will be tried for verbally and physically attacking another teen victim. The 16 year old abused victim was sent to the hospital and suffers from blurred vision‚ hearing loss‚ and a swollen face from the attack. The attack was meant to embarrass
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Available on the iPad THE 50 MOST INNOVATIVE COMPANIES April 15‚ 2010‚ 5:00PM EST The 50 Most Innovative Companies For the first time since Bloomberg BusinessWeek began its annual Most Innovative Companies ranking in 2005‚ the majority of corporations in the Top 25 are based outside the U.S. The reason: the new global leaders coming out of Asia By Michael Arndt and Bruce Einhorn In the past decade‚ as the U.S. was losing an estimated 2.4 million factory jobs to China‚ the Economic Policy Institute
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move And it say... Hahaha‚ yea And it go... That’s right I’m a millionaire I’m a Young money cash money fast money Slow money mo’ money neva no money What is that‚ who is that‚ I never heard of it I will take your picture and make a ’rest in peace’ shirt of it Someone should take this beat and make a rest in peace shirt of me because I Killed and now don’t tell no one you heard of me It’s like‚ the beat was screamin‚ murder me And I’m a‚ murderer So I murdered it And you rappers is
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Writing 121 12 November 2013 Attack of the 50 Foot Woman This cult classic B-movie‚ Attack of the 50 Foot Woman‚ provides one of the most comprehensive cross-section perspective of popular American society’s fears in the late 1950’s. With the country in turmoil‚ recovering from the recent WWII and gearing up for an impending war with Russia‚ Americans were fearful for their future. Their fears weren’t just of an attack from the silent north‚ but also of the importance of females in the workplace
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nd nd 2 INTERNATIONAL CONFERENCE ON BUSINESS AND ECONOMIC RESEARCH (2 ICBER 2011) PROCEEDING WHAT IT TAKES TO BE A MANAGER: THE CASE OF MALAYSIAN FIVESTAR RESORT HOTELS Rozila Ahmad (rozila@uum.edu.my) Noor Azimin Zainol (azimin@uum.edu.my) Universiti Utara Malaysia Sintok‚ 06010 Kedah ABSTRACT This article investigates the requirement for managerial posts in five-star resort hotels in Malaysia. Despite the growth of hotel industry in Malaysia‚ academic literature especially qualitative
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that it works for the ballroom dancers but not for the gangbangers‚ who continue on their chosen careers. There is a more pessimistic view of urban high schools in another movie opening today‚ "American Gun‚" and I fear it’s closer to the truth. But "Take the Lead" is said to be based on a true story‚ it tells a heartening fable‚ and Antonio Banderas is uncommonly charming as a dance teacher who walks into a high school and announces that he will improve it by his very example. Public manners have
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CLBC DAY PROGRAM QUARTERLY REPORT AUG/SEPT/OCT 2013 Individual: Arren Nash Keyworker: Sandy Hossack Health Update During this reporting period Arren has not had any physical heath issues to report. There were no toileting issues. Due to Arren’s increase in behaviors he was taken to a psch. Named Dr. Price on Sept. 11 and was given a new medication called Quetiapine. This was increased by 25mg on Oct.15/2013. Currently on 50mg. Social‚ Recreation‚ and Community Arren’s behaviors became extreme
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Occupy Wall Street Professor Craig Business Ethics May 5‚ 2013 “Occupy Wall Street is a leaderless resistance movement with people of many colors‚ genders and political persuasions. The one thing we all have in common is that We Are The 99% that will no longer tolerate the greed and corruption of the 1%. We are using the revolutionary Arab Spring tactic to achieve our ends and encourage the use of nonviolence to maximize the safety of
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In Questions 50-52 and 71 by Aquinas‚ he discusses habits and how a habit is a “disposition of a subject which is in a state of potentiality either to a form or operation” (ST I-II‚ q.50‚ a. 1). Based on this definition‚ it shows how people are morally responsible for their habits because even though it is a disposition‚ it has the potential to form the habit‚ but it has not actually happened yet without the person’s choice. That’s an important thing to remember because sometimes habits become so
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