The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
Premium Enzyme PH Chemical reaction
Title of Experiment: The Effect of Temperature on the Hatching Success of Brine Shrimps. Abstract In this experiment‚ 40 brine shrimp eggs are put into a test tube containing salt water at different temperature such as 25°C and 35°C respectively. The eggs are left from two days to hatch. After two days‚ the number of eggs successfully hatched are observed and counted. Introduction Brine shrimp is the common name for a primordial crustacean that reaches less than 1.3 cm in length and is usually
Premium Crustacean Arthropod
Aim: To find the effect of temperature on enzymes‚ using a potato as a catalyst. The source of catalase is in the potato cells. 2H2O2 → O2 + H2O Planning: Introduction: An Enzyme is any one of many specialised organic substances‚ composed of polymers of amino acids‚ that act as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organisms Enzymes are classified into several broad categories‚ such as hydrolytic‚ oxidising‚ and reducing‚ depending
Free Chemical reaction Enzyme Catalase
Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
Premium Catalase Enzyme Gas
enough to fit your hand) ice (not included in the lab kit) thermometers Part 2 test tubes – use three similar glasses or cups water various household materials for insulation stove or microwave to heat up water Part 1: Heat transfer and human sensation 1. Pour warm water in the bowl on the left‚ cold water in the bowl on the right‚ and room temperature water in the bowl in the middle. Room temperature water should be around 20-22°C. 2. Put your left hand in the warm water bowl and
Premium Temperature Heat Insulator
PART B YOU WILL NOW DESIGN AN EXPERIMENT TO STUDY THE EFFECT OF A FACTOR AFFECTING VITAMIN C IN FRUIT JUICES WHAT IS THE EFFECT OF TEMPERATURE ON VITAMIN C IN FRUIT JUICES? The purpose of this experiment is to determine if the difference in temperature will damage vitamin C in any type of fruit juices. Five different temperatures of fruit juices will be used(5‚ 22‚ 50‚ 70‚ 90) degree Celsius to see how temperature affects the loss of vitamin C in fruit juices. The amount of vitamin C in the fruit
Premium Vitamin C Juice Temperature
BAR CHART tab and turn on Show numerical values. 1. What is the initial temperature of each beaker? Beaker A 95 Beaker B 5 2. Click Play ([pic]) and observe. A. What happens to the temperature of Beaker A over time? Beaker a goes down over time. B. What happens to the temperature of Beaker B over time? Beaker b goes up. 3. Why do you think the temperatures of Beaker A and Beaker B changed as they did? Because of conduction. |Activity A:
Premium Heat Heat transfer Temperature
objective: In this experiment‚ I decided to find out how temperature would affect endothermic reactions compared to exothermic reactions. An endothermic reaction absorbs energy in the form of heat. This reaction feels cold because it is taking heat away from its surroundings. It also releases gases. An exothermic reaction is a reaction that releases energy as heat. Since the energy is released‚ the exothermic reactions feel hot. Methods/Materials I did three experiments. I mixed lemon juice
Premium Temperature Chemical reaction Thermodynamics
Biochemistry CP2085 Effect of Temperature‚ Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds
Premium Enzyme Energy Kinetic energy
For this experiment‚ there will be an optimal point for each factor. I believe that the optimal temperature for the enzyme sucrase will be 37°C because that is the point where the temperature increases the rate of reaction to its greatest point without denaturing it. For the pH test‚ I think that the optimal pH for sucrase will be the pH of 7 because it is neutral so it won’t affect the charge in a negative or positive way. For the denaturation test‚ I think that the optimal treatment for the enzyme
Premium Enzyme Temperature Glucose