with the eluent that is lower in polarity. 2. TLC in today’s experiment stands for Thin Layer Chromatography. 3. From least polar to the most polar: Heptane-Toluene-Acetone-Methanol-Acetic acid ‚ ‚ ‚ ‚ 4. The Rf (Retardation factor) is the ratio of the distance that the compound/mixture traveled to the distance the solvent/mobile phase traveled. 5. From least Rf to the highest Rf: Benzoic acid – Benzaladehyde - n-Decane If the TLC were run with a more polar solvent‚ all the
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Experiment C: Identification and Separation of Dyes by TLC Pre-lab Properties: Ethyl acetate‚ ethanol‚ silica‚ azobenzene‚ azulene‚ 4-(p-nitrophenylazo) resorcinol‚ methyl red‚ bromocresol green (solubilities in water and ethanol) Purpose: To identify compounds from an unknown mixture using TLC Up to 100% of missed points can be recovered from this lab Watch the video: http://www.youtube.com/watch?v=e99nsCAsJrw (MIT) TLC plates are near the main hood DO NOT BREAK CAPILLARY TUBES Keep spots small
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chromatography (TLC) is an extremely valuable analytical technique. It provides a rapid separation of compounds‚ and gives an indication of the number and nature of the components of a mixture. TLC can also be used to identify compounds by comparison with known samples‚ to check the purity of a compound‚ or to monitor the progress of a reaction‚ an extraction‚ or a purification procedure. This experiment will introduce you to the mechanics of TLC‚ and the chemical principles behind it. TLC is normally
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Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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IDENTIFICATION OF UNKNOWN SOLUTIONS USING TLC CHROMATOGRAPHY Purpose: To become familiar with the principles and terminology of TLC chromatography to identify an unknown molecule based on comparisons with known laboratory standards and Rf factors. Background Thin Layer Chromatography: Chromatography is a sophisticated method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that
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separated using column chromatography. Column chromatography is a separation technique that is used among many disciplines including biology‚ biochemistry‚ microbiology and medicine. Many common antibiotics are purified by column chromatography.1 Column chromatography allows us to separate and collect individual compounds. In this experiment‚ lumen will be the stationary phase‚ and the more polar substance will be retained on the stationary phase longer. To start out the experiment‚ a short pipette
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FUNCTIONAL FOODS & NUTRACEUTICALS LABORATORY REPORT LAB 2: FAT & OIL FROM OIL-SEEDS: PART II THIN LAYER CHROMATOGRAPHY ANALYSIS (TLC) COORDINATORS: ASSOC. PROF. DR. JAMALUDDIN BIN MOHD DAUD ABDUL AZIM ADNAN 1016741 INTRODUCTION TLC chromatography or thin layer chromatography is a type of planar chromatography. TLC is routinely used by researcher in the field of phyto-chemicals‚ biochemistry etc. to identify the components in a compound mixture like alkaloids
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Abstract In order to experiment with distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further
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Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography. Lab #10-2 Theory: Ethyl Acetate-Solvent used in TLC (mobile phase)
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Extension of a Rubber Band – Experiment Apparatus: Retort stand (with clamp) Spring (with hook) Masses (50g) Meter ruler Setsquare Rubber Band Method: 1. Assemble apparatus as shown‚ making sure the meter ruler is absolutely vertical‚ and that the clamps of the retort stand are tightened enough to hold the apparatus in place. 2. Measure the initial length of the rubber band‚ without any masses on it to get a reading at 0g‚ using the setsquare (place vertically against the ruler and read off it from that)
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