Abstract This experiment is to study and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient
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pubs.acs.org/joc Mechanism and Regioselectivity of the Osmium-Catalyzed Aminohydroxylation of Olefins Dominik Munz and Thomas Strassner* Physikalische Organische Chemie‚ Technische Universit€t Dresden‚ Mommsenstrasse 13‚ 01062 Dresden‚ a Germany thomas.strassner@chemie.tu-dresden.de Received November 12‚ 2009 The mechanism and regioselectivity of the osmium-catalyzed aminohydroxylation of olefins was investigated in detail by density functional theory (B3LYP/6-31G(d)) calculations in
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Part I: Reaction between iron and oxygen Appearance of steel wool after 20 minutes From (relatively) shiny metal strips to notsoshiny strips that are stained with dark‚ reddishbrown stuff. Part II: Decomposition of hydrogen peroxide Appearance of potato and hydrogen peroxide combination: Lots of white‚ foamy bubbles around the potato slices. Day 2 Observations: Part III: Reaction between zinc and acetic acid Appearance of penny in vinegar after 30 minutes: Pretty dark coloured
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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Title: Kinetics: The Rate of a Chemical Reaction Objectives: 1. To study the kinetics of chemical reaction‚ 2 I- + S2 O82- I2 + 2 SO42- . 2. To study the effects of reactant concentration (persulphate‚ S2O82-‚ and iodide‚ I-) and temperature on the rate of chemical reactions. ( i) Study the effect of 0.20M (S2O82-) on the rate of chemical reaction. ( ii) Study the effect of 0.10M (S2O82-) on the rate of chemical reaction. ( iii) Study the effect
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Reetika Kashyap September 20th‚ 2012 Enzyme Lab What is an enzyme? Enzymes are specialized protein molecules simplifying most of the body’s metabolic processes such as‚ supplying energy‚ digesting foods‚ purifying your blood‚ executing the body of waste products etc. Enzymes act as catalyst by speeding up the reactions that happen in our bodies and decreasing the amount of activation energy needed to break a complex down. A reactant is any given enzymatic reaction is called a substrate for that
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Introduction In this lab‚ we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The chemical reaction is shown as 2H2O2 = 2H2O + O2 (Keilin and Hartree 397). The reaction involves primarily the adsorption of hydrogen peroxide at the catalase surface. The decomposition of hydrogen peroxide by catalase is regarded as involving
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Chemical Reactions Lab Synthesis Reactions: Synthesis reactions occur when two elements or compounds combine to create one single compound. The general equation of synthesis reactions is: A+B→AB. The following are the complete balanced equations for the five synthesis reactions performed in the lab. Reaction 1: Reaction 2: Reaction 3: Reaction 4: Reaction 5: The reaction of CO2 and water is a prime example of a synthesis reaction. This is a synthesis reaction because it follows the general
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types of forces involved. Two of the main forces experienced are called potential energy and kinetic energy. Potential energy is the energy an object has depending on the position of it. For example the higher an object is the higher the potential energy is and the lower an object is the less potential energy it will have. Kinetic energy is also a main type of force that is involved with rollercoasters. Kinetic energy is the energy an object will have as it is in motion. There are many different types
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momentum of the object over a long time D. Increase the momentum of the object over a long time 8. Most street barriers are made of hard rubber or plastic materials that easily give in or dent when hit. Why is this so? A. To reduce the vehicle’s kinetic energy when it collides with the barriers B. To store potential energy in the bumpers of vehicles C. To reduce the impact force when hit by a vehicle D. To reduce the impact time when the barriers hit the vehicle 9. What is the momentum of a 20
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