Enzyme-Controlled Reaction Procedure: Click the TV/VCR. Then click the Play button on the video controller. Watch an animation about enzyme action. Click More Information to read about enzymes and substrates. To conduct the experiment: Adjust the pH level of the test tube by click the up and down arrows Add substrate to each of the test tubes that already contain an enzyme solution Click and drag a piece of weighing paper with the powdered substrate to a test tube. Click the computer monitor to
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____________________ Immobilizing an enzyme provides various analytical benefits‚ and can be done in a myriad of ways‚ with the most common being entrapment. For this study peroxidase (from horseradish)‚ an enzyme that catalyzes the cleavage of hydrogen peroxide into water‚ was entrapped within a polyacrylamide gel matrix. The gel matrix was formed by the addition of methylene bis-acrylamide (a cross linking agent) to acrylamide. The immobilized enzyme was then tested via spectrophotometric assay
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the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation‚ you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed reaction? Materials
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Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
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This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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Austin Vandermey GS-1140 April 4th‚ 2015 Experiment We as a society have one big problem on our hands. This problem is sleep deprivation because there are not enough hours in the day to get stuff done. Between work‚ school‚ family‚ and leisure it seems like we do not have enough time to get are daily tasks done let alone have time for sleep. It is recommended by the U.S Department of Health that people need a solid eight hours of sleep to maintain good health. I don’t know about you
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Experiment #1: Introduction to Experimentation Submitted by: Neann Klara M. De Jesus BS Psychology II Submitted to: Dr. Geraldine E. Tria ABSTRACT SUMMARY The first experiment done by the class was called “Introduction to Experimentation”. Its main objective is to give basic knowledge about some of the logic of experimentation. The class was divided into groups of 2. In each group there was an experimenter (E) and a subject (S). The experimenter instructed
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Purpose: The purpose of this lab was to determine the freezing point of pure water‚ the experimental van’t Hoff factor for a substance and the molar mass of an unknown solution. Reagents: 25mL deionized Water ¼ to ½ inches of rock salt 25mL Sodium Nitrate (NaNO3 ) 25mL unknown solution – handle unknown solution carefully Procedure: For part 1‚ the freezing point of deionized water was determined. An ice bath was prepared by filling a 600mL beaker ¾ full of ice and covered with ¼ to
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LAB REPORT FOR EXPERIMENT #2: PURIFICATION OF ACETANILIDE BY RECRYSTALLIZATION Your name TA’s name Your Partner’s name Lab Section OBSERVATIONS: A. SELECTING A RECRYSTALLIZATION SOLVENT | |Solubility Test (cold) |Solubility Test (hot) | |Water |insoluble |soluble | |pet ether |insoluble
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