Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen. Hypothesis: For this experiment‚ antibodies will be placed in wells
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1. Animals Experiments were carried out in adult male Wistar rats (200-225 g; 2-2.5 months old)‚ obtained from Central Animal Research Facility‚ NIMHANS‚ Bengaluru. Adult rats were group-housed (3 per cage) in a climate-controlled vivarium with a 12:12 h dark/light cycle. All animals had free access to food and drinking water except during the stress procedure or behavioral evaluation. All the experiments were performed during the light phase between 10:00 h and-14:00 h. All experiments were approved
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I.Purpose The purpose of this lab is to show how potential energy and kinetic energy is shown and transferred using a model rollercoaster. This lab also demonstrates the Law of Conservation of Energy. II.Introduction Potential and Kinetic energy have a very big relationship. The Law of Conservation of Energy states that “Energy cannot be destroyed or created‚ but can be transformed or transferred.” This lab will help demonstrate this law and show the conversion between Kinetic and Potential
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A Report of Experiment 1 Purification and Identification of An Unknown Mixture Aim To separate a mixture of a neutral (ester‚ alcohol‚ ketone‚ aldehyde or ether) and an acidic compound (phenol or carboxylic acid) or a neutral and a basic compound (amine)‚ and to identify the unknown mixture by physical (M.P. or B.P.) and spectroscopic methods (NMR‚ IR and MS). Method The mixture (sample number: 27) was dissolved in 200 mL of CH2Cl2 and then extracted with 10% NaOH. The acid was removed
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and pestle Graduated cylinder Distilled water Test tubes Chicken liver (or any other liver) Sterile transfer pipet (Dropper) Sterile weigh boat Hydrogen peroxide Razor blade Cutting board Electronic balance Timer Ruler Clean everything Calculate mass of chicken liver needed for 1ml‚ 5ml‚ 10ml‚ 15ml‚ and 20ml catalyzes concentration mass to volume concentrations. In other words: 1% = . So‚ 15ml and H2O x = 0.15 g liver. This means 0.15g of liver for each percent of catalyze concentration
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Virtual Lab: Classifying Using Biotechnology Worksheet NOTE: As you read the information in the Microbiology Handbook‚ there may be some terms you are not familiar with – such as 16s ribosomal RNA and Polymerase Chain Reaction. Refer to your text to read background material explaining any terms or processes with which you are not familiar. Record the results of your investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first
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Derricka Joe Period 5 1 Oct.‚ 2014 Lab Experiment One: Physical Properties Lab Analysis: For our first unknown‚ we observed the color and odor of the substance. The substance was a clumpy‚ white‚ crystal-like substance and it smells like new plastic. When we tested the solubility in water it resulted insoluble and in ethanol it resulted soluble. This substance could not be tested for electrical conductivity because the water was insoluble. We then tested for the PH value and it was neutral. The
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