Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas species. If the Pseudomonas species are present‚ a blue-green or brown pigmentation under the daylight‚ or fluorescence can be taken as a presumptive evidence under UV light. In
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Goal(s): To study the effect of concentration on the rate of a reaction‚ and to find the order of the reaction in each reactant and write a rate equation. Background Theory: Chemical reactions occur at different rates that ultimately depend on their concentration‚ the temperature‚ the usage of catalysts‚ the nature of reactants‚ like the surface area of the particles. Concentration‚ for instance‚ increases the number of molecules or particles in a certain volume‚ so collisions will become more
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oxygen‚ carbon dioxide‚ helium‚ and lab gas. We took a Ziplock bag‚ and turned into a non-stretchy balloon‚ and filled with each gas and measured the apparent mass then calculated the actual mass‚ then find a ratio between the mass of oxygen and the other gasses. We tried to keep the pressure about the same each time so our calculations would be more accurate. We found out that the heaviest was carbon dioxide‚ and the lightest was helium. In fact‚ helium and lab gas had a density below 0. Anyways
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Celestial Geography I Emily Richarz PH090-04 Purpose: The purpose of this lab was to introduce us to star charts and how to use them to find stars‚ constellations and other bodies in the sky. Procedure: The instructor gave a brief but thorough lecture and demonstration on how to go about finding the answers on the worksheet. We then filled out the worksheet that cover finding stars and constellations using right ascension and declination in general and on specific dates‚ where constellations
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Department of biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris LABORATORY REPORT SBT 1013: INTRODUCTION TO BIOTECHNOLOGY Semester 4 Sessions 2013/2014 ID NUMBER AND NAME 1. SITI NURFATINI BINTI MOHAMAD ROSLAN (E20131007560) 2.NURHAFIZAH BINTI SAMSUDDIN (E20131007584) 3.MUNIRAH BINTI HARON (E20131007556) 4.NORSYAWANI BINTI SUPIAN (E20131007577) LECTURER : CIK FATIMAH EXPERIMENT NO. : EXPERIMENT NO.1 TITLE : EXTRACTION OF DNA
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Wanni Lin Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are
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Lab: Flame Test Purpose: to determine the ID of 2 unknown substances Background Information: Every atom consists of a nucleus with tiny electrons whizzing around it. The further away from the nucleus they are‚ the more energy the electrons have. If a metal atom is heated‚ the electrons get enough energy to jump higher away from the nucleus‚ they become “excited”. When they fall back closer to the nucleus (back to their ground state)‚ they give off this extra energy as light. Why is the
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successfully synthesis the reactant to the product‚ NaBH4 was used as the main reagent to reduce the carbonyl double bond. One believes the formation of isoborneol was successfully due to the product’s percent yield‚ IR‚ and melting point. During the lab‚ .077 grams of isoborneol was yielded from the camphor reduction. In result‚ .077 g compared to an theoretical yield of .102 g equaled an overall 75% percentage yield. Considering the product yield was only a quarter shy of a 100% yield‚ provides strong
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distributions are unknown. To explore the putative hyperdiversity of tropical leaf endophytes‚ we compared endophyte communities along a broad latitudinal gradient from the Canadian arctic to the lowland tropical forest of central Panama. Here‚ we use molecular sequence data from 1403 endophyte strains to show that endophytes increase in incidence‚ diversity‚ and host breadth from arctic to tropical sites. Endophyte communities from higher latitudes are characterized by relatively few species from many different
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