Introduction Microscopy is an essential technique not only in cell biology but natural science as a whole. We compared different types of microscopic techniques ‚ according to the specimen used and the scope of the experiment. Two specimens‚ stained and unstained‚ containing CHO cells‚ were prepared‚ examined and analyzed under the microscope using bright field (HF)‚ dark field (DF) and phase contrast (PH) settings. In addition‚ the four phases of cell division cycle were estimated.. Bright field microscopy
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Effects of Temperature on the Formation of Cheese Curds This lab was performed in order to find what temperature the enzyme chymosin is most effective at forming cheese curds. An enzyme is a protein that acts as a catalyst to speed up biological reactions in cells. They work by lowering the activation energy and help to orient the molecules correctly. Chymosin was originally taken from the stomachs of calves‚ and was called rennet‚ and then purified to make rennin. Eventually to catch up with
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F = ρgXA Where: ρ = water density g = acceleration due to gravity X = vertical distance from free surface to centroid of A We know that the magnitude of the distributes force F‚ which may be considered as a small series of small forces spread over the submerged surface. The sum of the moments of all these small forces about any point must be equivalent to the moment about the
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Coli bacteria. We began the lab by first obtaining two sterile microcentrifuge tubes‚ one was label “GFP+” while the other one was “GFP–” because this one served as a control meaning nothing goes inside this one. With gloves on 25 microliters competent cells‚ which is e. Coli bacteria compete in calcium chloride and heat shock was added to each of the microcentrifuges. Eventually‚ both of the
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General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
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Anxiety Lab Report Abstract: The aim of this study is to investigate the relationship between cognitive anxiety‚ somatic anxiety and self confidence in two different situations; non-stressful and stressful. It is to examine the effects on anxiety of a simple golf putting task. The stressor used in this experiment can be described as situational‚ namely the stressor of social comparison‚ the type of stressor that leads many performers to question their own ability which in turn evokes symptoms
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Purpose: The purpose of this lab was to see how the changes in exercise intensity affect the rate of metabolism within the body. Methods: In this lab‚ indirect calorimetry was used to measure metabolic rate by calculating caloric expenditure by the measurements of oxygen consumption. The variables measured were the fraction of oxygen expired‚ the fraction of carbon dioxide expired‚ and the total volume of air inspired. This experiment required the participation of two volunteers. First‚ the variables
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Ph Lab Report Bryon Kim 123013 B(2) Biology Background information/Research PH paper (litmus paper) determines how acidic or how basic a substance is. The paper changes color accordingly to color code on the pH scale. The pH scale starts from 0 to 14. The lower the number the more acidic it is. Zero is the most acidic‚ and 14 is the most basic while 7 is the neutral number for example water. Examples of an acid is lemon juice or multi purpose cleaner. Examples of a basic substance is shampoo or liquid soap
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original state and the percent of the hydrate recovered was calculated by using the mass of the rehydrated sample by the mass of the original hydrate and then multiplied by 100%. Data Presentation & Analysis Table 1: The data was collected from the lab experiment. Sample calculations are shown. Mass of beaker with sample 30.765g Mass of empty beaker 30.263g Mass of sample .502g Mass of beaker with sample after 1st heat 30.661g Mass of beaker with sample after 2nd heat 30.657g Heating mass
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Maryely Ramirez Professor Theresa Sterner Bio Lab 105 04 November 2013 The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions‚ such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva‚ and pancreatic secretions of the small intestine. The function of amylase is
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