Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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Chemistry lab report Experiment 32 Voltaic Cell Measurements Dinmukhamed Yeraly Partner Azamat Bashabayev General Chemistry II lab Instructor Rostislav Bukasov Nazarbayev University Introduction Most of the chemical reactions can be classified as redox reactions‚ which include two half reactions‚ oxidation and reduction respectively. To measure the tendency for a readox reaction to occur‚ special apparatus called voltaic cell can be used. It is made by connecting a voltmeter between
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Chemistry Lab Report Water Hydration Introduction: A hydrated crystal or hydrated occurs when water becomes tightly attracted to a metal salt base on it’s polarity. The water molecules maintain integrity as molecules‚ however they are considered to be part of the formula of the hydrate. When the hydrate metal salt crystal is heated‚ the attractions to the water are broken by the heat energy and the water escape from the crystal. After heating the salt crystal is called as anhydrous‚ which
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Tim Readmond AP Biology Lab Report I. Title a. Modeling Diffusion and Osmosis II. Introduction a. If one places a 1.0 M solution of glucose inside a bag and then places that bag into a beaker containing a 1.0 M solution of sucrose‚ the percent of mass lost in the bag is 10.5%. The solution in the bag is hypertonic while the solution in the beaker is hypertonic‚ which is why water moves from the bag to the beaker and the bag loses mass. b. The purpose of this experiment is to see whether
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Lab Report An enzyme is a protein that speeds up the rates of chemical reactions. They recognize‚ bind‚ and change specific reactants. They do not change so they can catalyze the same reaction again and again. Activation energy is the amount of energy needed in order to begin a chemical reaction. A Catalyst is a substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. Catalysts are substances or a substance that configures another substance
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allowing water‚ glucose‚ salt and waste materials to pass through to the Bowman’s capsule but preventing and red blood cells and plasma proteins to pass through. Reabsorption occurs in the proximal tubules of the nephron. Water‚ glucose‚ amino acids‚ sodium and other nutrients are reabsorbed into the bloodstream in the capillaries surrounding the tubules. Water moves via the process of osmosis: movement of water from an area of higher concentration to one of lower concentration. Tubular secretion is
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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factors of a chemical reaction affect the rate of reaction. This experiment focused on how temperature affects the rate of reaction for the reaction of alka-seltzer and water. The rate of a reaction is a value calculated to figure out how fast a chemical reaction occurs. There are three factors that can be changed that will affect the rate of a chemical reaction: surface area‚ concentration‚ and temperature. Temperature specifically affects the rate of a chemical reaction‚ because when the temperature
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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