manner that was similar to the method of amplexus males use in order to release the eggs. The released eggs were placed in a petri dish where they were to be fertilized by the sperm of a male frog‚ who had donated his life prior to the running of the IVF treatment. In order to fertilize the eggs‚ the testis was diced into a small piece and then was moved around the petri dish touching every egg present in hopes of fertilizing each one. The eggs were then left for five minutes before adding a simple
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Practical Assessment 2 – The effect of substrate concentration on the rate of enzyme activity of Catalase Aim To investigate the effect of substrate concentration (manipulated by increasing concentration of hydrogen peroxide) on the rate of enzyme activity of catalase‚ produced by liver cells‚ on the decomposition of hydrogen peroxide. Introduction Enzymes are biological catalysts that increase the rates of reactions. In an enzyme-catalyzed reaction‚ the substrate binds to the active site
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A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
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Purpose: The purpose of doing this lab was to observe the regenerative patterns of planaria cells after a portion of their bodies was cut off. Background: 1. A blastema is a group of cells that forms at the site of a wound that eventually turns into the missing parts of a planaria. 2. Planaria fission occurs when a planaria is missing body parts. The organism attaches its tail-end to the ground and pulls itself forward using its head-end in order to tear itself into two parts. Then‚ each of these
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------------------------------------------------- ------------------------------------------------- EVALUATION OF EFFECTIVITY OF SOAPS AND DETEGENTS AGAINST BACTERIA BACTARIUM EVALUATION
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Exercise 1: Correct Use of the Scientific Method and an Experiment with Macromolecules I. Objectives In this lab‚ students will: a.) Learn how to utilize the Scientific Method to develop a testable hypothesis b.) Generate an effective experimental design from a hypothesis c.) Understand the chemical differences between different macromolecules and how these differences can be used to design tests for each molecule. d.) Identify macromolecules in solutions using chemical analyses. e.) Practice
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What we are going to test on. Petri dishes- Transparent glass cylinder which holds 100 mL of liquid. Used for holding seeds. Paper towels- Corse paper which was greyish in color. Used for putting seeds on and cleaning up messes. Water- Transparent liquid with no taste‚ odour
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was to determine the effects of different antimicrobials on both Gram-positive bacteria and Gram-negative bacteria. Procedure Overall‚ exercise 15 was broken down into two days of procedures. For the first part of the experiment‚ we divided three petri plates into three equally sized units on the plate and marked them with wax pencils in accordance to which antimicrobial infused disk would be placed there. Following this‚ we aseptically poured the three plates with nutrient agar. Since we were assigned
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flatworms due to their embryonic stem cells called neoblasts. We obtained the flatworms used in this experiment by first placing a few drops of spring water on a petri dish so that the flatworms will be able to survive a couple of weeks‚ and not be dehydrated. Next‚ we used a pipette to gather the flatworm and then placed the worm on the petri dish‚ with the spring water. Using a razor blade‚ the worm was cut in half into two sections of 6mm in length and 5 mm in length. The worms were measured using a
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E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked‚ and then a small amount of the contents were extracted and placed into two petri dishes containing ampicillin (hinders growth of bacteria). After a couple of days‚ the petri dish without P Vib displayed no signs of colonization in the E. coli
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