stained if the dyer solution is used at the specific pH at which the reactive groups are fully ionized. To achieve full ionization of the reactive groups‚ some alcian blue methods begin with a rinsing of sections in the appropriate pH solution prior to staining with the dye solution. In general‚ strongly sulfated mucins react more consistently at low pH levels. Above a pH of 1.0 their reactions are variable. At a pH of 0.2 only strongly sulfated mucosubstances will be demonstrated‚ while weak sulfated mucins
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ESSAY ON IDENTIFYING A PEER REVIEWED PRIMARY RESEARCH ARTICLE [Main body word count 512] Journal Location: http://mic.sgmjournals.org/content/158/2/353.full Microbiology 2012 February; 158(Pt 2): 353–367. doi: 10.1099/mic.0.053371-0 The purpose of this report is to describe a peer-reviewed journal article that has been identified from a primary literature source. I chose to perform a search through Pubmed based on the enzyme Catalase‚ reasoning that it should deepen my understanding of the
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This technique was called staining‚ and was when one would stain nervous tissue before examining to under a microscope. However‚ several years later in 1873‚ Camillo Golgi came out with a new‚ improved staining method. The staining technique‚ allows the frequently transparent microbial cytoplasm’s to be seen with the light of the microscope by staining them. Through this new method there were several new discoveries‚ surrounding the nervous
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beat. Greenley’s Painting provides painting services in Sussex‚ Kent and New Castle Counties of Delaware and in parts of Maryland. They offer a wide range of painting services such as: foundation painting‚ oil based painting‚ metal painting‚ gel staining‚ epoxy floors‚
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Bacteria Morphology April 2‚ 2015 Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. Hypothesis – The experiment will allow for further insight into stained organisms‚ allowing extended contrast‚ differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens
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Borrelia burgdorferi Overview: Lyme disease was first recognized in the United States in 1975 by Dr. Allen Steere‚ following a mysterious outbreak of juvenile rheumatoid arthritis near the community of Lyme‚ Connecticut. The rural location of the Lyme outbreak and the onset of illness during summer and early fall suggested that the transmission of the disease was by an arthropod vector. Adult female Ixodes tick (deer tick) From Brown University In 1982‚ the causative agent
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Part I INTRODUCTION TO MICROBIOLOGY 1 The Evolution of Microorganisms and Microbiology CHAPTER OVERVIEW This chapter introduces the field of microbiology and discusses the importance of microorganisms not only as causative agents of disease‚ but also as important contributors to food production‚ antibiotic manufacture‚ vaccine development‚ and environmental management. It presents a brief history of the science of microbiology and an overview of the microbial world. The origin of life and
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EXPERIMENT 1: PREPARATION OF BIOLOGICAL MATERIALS FOR MICROSCOPIC EXAMINATION Introduction: Cell is the smallest structural and functional unit of an organism‚ and is often called the building blocks of life. All organisms are composed of one or more cells‚ they are classified into 2 groups which are unicellular organisms and multicellular organisms. Plants and animals are made up of cells too. There are two basic classes of cells‚ prokaryotic and eukaryotic cells. The structurally simpler‚ prokaryotic
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consists of pili (Todar‚ n.d). The pili on this pathogen work with the toxin‚ adenylate cyclase‚ to bind Bordetella pertussis to human respiratory epithelium (Hartsock‚ n.d.) While this pathogen does produce toxins‚ it does not form endospores (Finger & von Koenig‚ 1996). Endospores allow cells to survive in very complex environments‚ and they are dormant cells. This means that Bordetella pertussis isn’t capable of surviving in harsh environments. Spores are
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Yun-Zheng Le‚2 and Alfred S. Lewin1 1 Department of Molecular Genetics and Microbiology‚ College of Medicine‚ University of Florida‚ Gainesville‚ Florida‚ United States Departments of Medicine‚ Endocrinology‚ and Cell Biology and Harold Hamm Diabetes Center‚ University of Oklahoma Health Sciences Center‚ Oklahoma City‚ Oklahoma‚ United States 2 Correspondence: Alfred S. Lewin‚ Department of Molecular Genetics and Microbiology‚ College of Medicine‚ University of Florida‚ Box 100266‚ Gainesville‚ FL
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