intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33 Part A: Using the Swift M5 Microscope and Viewing a Letter “e” 34 Lab 3: Microscopy 39 In-Lab Report Sheet 39 Part A: Using the Swift M5 Microscope and Viewing a Letter “e” 39 Laboratory 4: Cell Structure & Function 43 Pre-Lab Assignment 43 This page left intentionally blank 44 Lab 4: Cell Structure
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that gives strong circumstantial evidence for the particulate nature of air. Materials: ✓ Smoke cell‚ incorporating a light source and lens (Whitley Bay pattern) ✓ Microscope‚ low power (e.g. x10 objective‚ x 10 eyepieces) and large aperture ✓ Power supply‚ 0 to 12 V dc ✓ Microscope cover-slip ✓ Smoke source (e.g. paper drinking straw) Setting up apparatus: [pic] The smoke can come from a piece of burning cord using a dropping pipette or
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sample mounting The paraffin section was cut at 5 μm of thickness with a rotary microtome. Sections of tissues obtained were floated on the surface of warm water in a floatation bath to flatten the sections. The sections were picked up onto the microscope slide‚ drained it in vertical position for several minutes. The slides were then placed on a slide holder in the oven at 37 °C overnight. Step 5: Staining The slides were placed in a staining rack and were dipped into xylene for 5 minutes
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experiment is conducted so that we may know how things happen in the level of cells. Objectives: 1. To demonstrate the principle of Osmosis and to apply it with actual life situations. Materials and Methods: • Materials: Compound microscope Glass Slide Cover Slip Tissue Paper Blade Rhoeo discolor Leaves Salt Solution • Procedures: Place a drop of distilled water on a clean glass slide. With the use of a blade‚ cut a thin slice of the lower epidermal leaf
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KARYOTYPING Karyotyping refers to use of a microscope to examine the size‚ shape‚ And number of Chromosomes in a sample of body cells. Extra‚ missing‚ Or abnormal positions of chromosome pieces can cause problems with a persons growth‚ development‚ and body functions. In karyotyping‚ the chromosomes in a cell are stained with a dye to make the bands of each chromosome visible. Banding helps show differences in structure among the chromosomes and helps arrange them into pairs. The chromosomes
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instruments such as microscope‚ staining‚ stethoscope and X-rays. Of the five senses‚ the most important is sight. It aids in the process of gathering information about the environment that we are part of. However‚ this visual gathering is adequate only to a certain point. Beyond this point‚ the unaided human eye fails to help us; the amount of detail that it can provide is severely limited. In order to overcome this limitation‚ humans started to develop instruments like microscope. The development
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their structure is linked to the development of microscopes‚ which allowed scientists to observe microscopic cells. In the mid 1600s‚ in the Netherlands‚ the scientist Antonie van Leeuwenhoek developed the first known microscope using a single magnifying lens. He is described as the first microbiologist because he was the first to observe microscopic cells that we now know to be bacteria and blood cells. Van Leeuwenhoek shared the designs of his microscope‚ as well as his observations‚ with the scientific
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_________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers of Magnification Available Type of Glassware that Specimens are Contained in... Is a 3-Dimensional View Possible? 3. Why is it not possible to perform dissection under a compound microscope? The focus is too high and the stage is too small 4. Why would a compound microscope be useful to a: a) bacteriologist? They use these devices to identify
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Your assignment is worth 50 points. Question 1 of 25 What property of a compound light microscope enables a person to change objectives without major focusing adjustments? resolving power magnification parfocal focusing resolution Question 2 of 25 How does closing the iris diaphragm affect image brightness and contrast? decreases both increases both decreases brightness and increases contrast increases brightness and decreases contrast decreases brightness but
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PRACTICAL 3 QUESTIONS PART 1 Q 1. What is the diameter of the field of view of your microscope using the x10 objective (i.e. at a magnification of x100)? The diameter of the field of view using the x10 objective is 1800 µm. Q 2. What is the diameter of the field of view of your microscope using the x40 objective (i.e. at a magnification of x400)? The diameter of the field of view using the x40 objective is 400 µm. Q 3. What is the ratio of magnification between the x10 and x40 objectives?
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