Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*‚ Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: September‚ 2010 ____________________________________________________________ _________________________________ Abstract: The experiment was done to separate and analyze the components of chili pepper. Chromatography was used because of its
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Tittle: Separation of leaves pigment. Introduction The photosynthetic pigment is used to absorb light during the process of photosynthesis. There are five pigments usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis
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titrate the NaOH into the solution. Start the data collection to calculate the volume of NaOH is added when the solution reaches its equivalence point. Then right down the calculations and disassemble the apparatus. Discussion My lab group and I completed this experiment doing two trials. We found that both trials were concluded with similar results. In trial 1 we found that the volume of
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Paper Clip Lab By: Daniel Del Carmen Mrs. McCormic IBH Biology 12 August 30‚ 2012 Background Information: You should find on some parts of your body you can feel the separate points when they are only a few millimeters apart‚ while for other parts of the body you may need a centimeter or more between them. When we touch something‚ it changes the shape of our skin slightly. We have nerve cells in our skin that detect these changes in shape and send a message to our brains‚ letting us
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Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3) M.P
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1 – Day Food Intake Paper Marisa Martinez SCI/220 7-10-2012 Dr. Angela Branch 1 Day Food Intake Paper Food intake is something that everyone should take very seriously in their daily lives. Food intake is regulated by the complex interaction of psychological and physiological events associated with ingestion. While the energy content of foods has an important role in determining the amount eaten‚ a number of other properties of foods also may be important
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Mineral Lab Paper Week One Mineral Lab Geology SCI/245 Axia College Mineral lab Paper The four requirements for minerals are that they must be naturally occurring in other words they must appear in nature something that that was formed by the earth and not man made. It must be inorganic which means I must not be formed from an organic substance like a fossil of any other living creature. It must have a specific crystalline structure that is formed from simple salts and complex silicates
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Biological Sciences College of Arts and Sciences Visayas State Unversity Visca‚ Babay City‚ Leyte Name: Millama‚ Engene O. Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment
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Experiment C: Identification and Separation of Dyes by TLC Pre-lab Properties: Ethyl acetate‚ ethanol‚ silica‚ azobenzene‚ azulene‚ 4-(p-nitrophenylazo) resorcinol‚ methyl red‚ bromocresol green (solubilities in water and ethanol) Purpose: To identify compounds from an unknown mixture using TLC Up to 100% of missed points can be recovered from this lab Watch the video: http://www.youtube.com/watch?v=e99nsCAsJrw (MIT) TLC plates are near the main hood DO NOT BREAK CAPILLARY TUBES Keep spots small
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different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance‚ followed by chlorophyll
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