By: Milton Antwi THE DIFFERENCES BETWEEN THE TWO MAIN TYPES OF BACTERIAL SPECIES The core difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria
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Cervical Cancer is a type of cancer that is formed in the tissues of the cervix‚ which is located between the lower part of the uterus and the opening of the vagina. Cancers develop when cells in the body get excited and grow out of control. A few things like having too many sexual partners or leaving in birth control for too long can factor into the development of cervical cancer but the majority of cases are due to a sexually transmitted disease (STI). Even though all women can run the risk of
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Screening Cervical cytological screening is accomplished by the Papanicolaou (Pap) smear screening test. Since this test was introduced in the 1970s‚ deaths resulting from cervical cancer have decreased by 71%.12 With this test‚ the patient is placed in the lithotomy position‚ and the provider inserts a speculum into the vaginal opening until the cervix and cervical os are visible. Then‚ the provider takes a cytological sample from the transformation zone of the cervix using a collection brush and
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globulins‚ alpha-2 globulins‚ beta globulins‚ and gamma globulins. Results: As shown in next page. Discussion: From what we observe in the gel results there are four bands and one smear‚ in seven lines which every line present a different serum (except 6 +7 are same 1 +2). Each band and the smear present different compound of serum protein. The first thick band is albumin which is the major fraction in a normal SPEP‚ and according to its concentration in serum it has the thicker band as
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world-wide. This disease is truly a scourge for our countrywomen. Cervical cancer can be detected in early or pre-invasive stages with the help of screening‚ thus greatly reducing the morbidity and mortality. In developed countries with an organized Pap’s smear screening program on a national level‚ the incidence and mortality from cervical cancer has been dramatically reduced. However‚ screening of all women in India is likely to remain an unrealized goal due to various reasons. So far‚ screening has not
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abnormalities‚ and formulate evidenced-based plans of care. In evaluating myself‚ I consider myself to have experienced substantial growth in knowledge‚ skill‚ and ability as a result of this clinical rotation. For instance‚ in performing my first Pap Smear‚ I was very nervous and was not smooth in my delivery of the service. After guidance from Ms. Rossow‚ I was able to perform several more‚ each time becoming more proficient. Similarly‚ as the time progressed‚ my assessments improved‚ enabling me to
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3.3 Microbiological Analysis 3.3.1 Bacteria count using Heamocytometer This method was used to determine the of three types of dessert but does not differentiate the type of bacteria. 25 g of food samples will be homoginesed in a sterile stomacher bag and shaken for two minutes with 225 ml of peptone water to obtain the food mixture. Using separate sterile pipets‚ decimal dilutions of 10-2‚ 10-3‚ 10-4‚ 10-5 will be prepared and others as appropriate‚ of food homogenate by transferring 10 ml of previous
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organism‚ I did a gram-stain to identify if the organism is gram positive or negative. I created two slides to ensure that I did the stain correctly with similar results. The gram-stain consists of creating a smear from the given plate and fixing it to the plate with methanol‚ then‚ dying the smear with crystal violet for
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First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the smear was sequence covered with crystal violet‚ iodine‚ 95% ethanol‚ and safranin; each reagent was rinsed with water after 1 minute. When decolorized with 95% ethanol‚ the smear was slowly
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Study guide for Microbiology Chapter 2 Name________________ 1. List and describe the sequence steps routinely used to identify bacteria. These are referred to as the “five I’s” in your text. 2. Define / describe each of the following as they apply to microbiology: a. Culture f. pure culture b. Inoculum g. contaminated culture c. Inoculation h. mixed culture d. Colony 3. Microbiologists employee a number of approached to acquiring a pure culture
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