The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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Preliminary Unknown Report Alexis Grettenberger 800503431 Katie Young Alexis Grettenberger Biol 311 Lab 11/21/14 Preliminary Unknown Report Purpose: The purpose of this experiment was to identify a specific unknown bacterium that was isolated from a multitude of bacteria in a sample of contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that
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2-Methylcyclohexanol February 10‚ 2013 Introduction This experiment was done to demonstrate the practical use of dehydration reactions and produce three different products. Dehydration reactions are reactions that involve the leaving of an “-OH” or “-H” group resulting in the formation of a double bond between two carbons. For the experiment we used 2-Methylcyclohexanol as a beginning reagent to dehydrate and possibly form 3-methylcyclohexene‚ 1-methylcyclohexene‚ or methylenecyclohexane. Drierite®
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contamination Purpose /Objective Objective: To test salmonella in spinach sample over five lab periods. Food contamination of salmonella can cause serious illness. Only small numbers of salmonella need to be found for a food product to be considered contaminated. Tests used Pre-enrichment- lactose broth Selective enrichment broth- with Tetrathionate Brilliant Broth Selective Plating- Brilliant Green Agar Isolation of salmonella conformation- is preformed using TSI and LIA Test results Tetrathionate
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Introduction The purpose of this lab was to demonstrate the use of gene therapy on diseases that are caused by a single gene defect. This procedure was demonstrated on two different strains of baker’s yeast‚ EAY 235 and EAY 431‚ which both contained mutations in the LEU2 and TRP1 genes. Neither of these strains will grow without a proper medium that would supply both of these essential amino acids. The EAY 431 strain of yeast also contained a Rad 52 deletion‚ which caused EAY 431 to be a deficient
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Research & Design in Psychology / G Faculty of Health‚ University of Canberra LAB REPORT COVER SHEET Instructions: 1. Complete these details and the declarations electronically. 2. Insert this sheet at the start of your lab report. 3. Submit the entire assignment‚ including this coversheet‚ as one file via the lab report drop-box on Moodle. 4. For more information‚ see Lab report guidelines. |STUDENT NAME:
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Formal Lab Report #1 I. Basics Title of the Experiment: The Empirical Formula of an Oxide Authors: Section Number: Chemistry Location and Date II. Abstract The aim in this lab was to determine the empirical formula of an oxide of magnesium through combustion in air. This was achieved by heating an established mass of magnesium in air inside of a crucible‚ ultimately attaining a compound that contained Mg and O. The major result of the experiment was the empirical formula of the oxide of magnesium
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The purpose of this lab is to successfully infiltrate E. coli bacterial cells with a pARA-R plasmid that is antibiotic resistant and has the rfp gene‚ or red fluorescent protein. This can be verified if the E. coli obtains the characteristics of the plasmid when it enters. To start‚ three Petri plates containing agar are needed. On each plate there is a control group and a treatment group; the treatment group being the one with the plasmid. Before the plasmid is put with the E. coli‚ first the bacteria
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A.‚ and Y. Will. 2007. The significance of mitochondrial toxicity testing in drug development. Drug Discovery Today. 12(18): 777-785. Galbraith‚ D.W.‚ D.P. Bourque & H.J. Bohnert. 1995. Isolation and fractionation of plant mitochondria and chloroplast. Methods in Cell Biology. 2: 161-173. Gross‚ V. S.‚ H. K. Greenberg‚ S. V. Baranov‚ G. M. Carlson‚ I. G. Stravovskraya‚ A. V. Lazarev & B. S. Kristal. 2011. Anal Biochem. 418(2): 213-223. Labbe‚ G.‚ D. Pessayre & B. Fromenty. 2008. Drug-induced
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INTRODUCTION In this lab‚ we will be measuring the viscosity of samples of polyvinyl alcohols in order to determine the molecular mass of their components. Polyvinyl alcohol is an example of a synthetic polymer‚ which is a macromolecule. The general chemical structure of such compounds are well-characterized‚ although variation in characteristics such as molar mass‚ chain length‚ and extent of branching are not obvious from merely looking at their molecular formula. There is also the complication
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