Winny Stephanie Experiment 1: Quantitative Determination of Protein Concentration Using the Biuret Test Table 1: Experimental protocol for construction of the protein standard curve Tube 1 2 3 4 5 6 Buffer (ml) 1.0 0.8 0.6 0.4 0.2 0.0 BSA Protein solution (10 mg ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table
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Protein 34 g 21: Salmon with lemon Servings: 4 Prep time: 10 minutes Cooking time: 30 minutes Ingredients: 6 ounces salmon fillets‚ skinless 1 pound asparagus ends trimmed 2 clove garlic‚ minced 3 tablespoon fresh lemon juice 3 tablespoon olive oil Salt and pepper to taste 2 lemon‚ thinly sliced Fresh thyme or dill sprigs Directions: Preheat oven to 400 F. In a bowl stir together oil‚ lemon juice and garlic. Season salmon and asparagus with salt and pepper. Place the salmon and asparagus
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Biochemistry of Proteins; Isolation of Ovalbumin and Enumeration of thiol groups Abstract Thiol groups are important to protein folding and forming disulphide bonds that are essential to protein structure. Determining the number of thiol groups in a protein is important in determining the tertiary structure of the protein. The ovalbumin is the experiment was purified from egg white using centrifugation and ammonium sulphate precipitation and then the thiol groups identified using DTNB and spectroscopy
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Final Essay | Analysis of the speech: Protein Pointers by Doctor Andrew Weil | | María Andrea Cevallos. | February 17‚ 2013 | | Abstract The objective of this essay is to describe a speech and to analyze five elements of the speaker’s verbal and non verbal communication. This essay contains three main parts. The introduction‚ that includes information about the speech‚ the central idea‚ a brief biography of the author and the year and place when the speech was released. The
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produce proteins? Intro(10mins) Bacteria belongs to a group of organism that lacks cell nucleus and membrane bound organells. This group of organisms are termed as prokaryotes. Prokaryotes follows the central dogma of molecular biology first proposed by Francis Crick in 1958 to synthesize proteins from mRNA through a process called translation and the mRNA is being synthesized from the DNA by another process called Transcription. Temperature‚ nutrient availibity are some key factors that
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Biol 228 Virulence factors of Legionella pneumophila The Gram-negative bacterium Legionella pneumophila contains a singular monopolar flagellum which is composed of a major subunit‚ the FlaA protein. Motility is associated with the infectious phase of L. pneumophila. In the initial phase‚ the replicative phase‚ the bacteria are immotile and have nonexistent or low toxicity. The growth of flagella leads to the infectious phase‚ where the new motile form of L. pneumophila is highly toxic and
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TABLE OF CONTACTS • Introduction……………..……..………………………....…………..……3 • Why we are choosing this issue (Thalassemia)?……….………………..…3 • What is Thalassemia?………………………………….…………………...3 • Types of Thalassemia……………………...……...….…………………….3 o Alpha Thalassemia…………..………………....………………….….3 o Beta Thalassemia…………………………..…………………………4 • Treatment of Thalassemia………………………….……………………….4 • The effects of Thalassemia………………………...……………………….4 • What can
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is known to present cell-surface proteins that are involved in the process of adhesion to mammalian epithelial cells which in turn contributes to its virulence. The Als (agglutinin-like sequence) family are encoded by eight gene loci‚ Als1-Als7 and Als9 (Murciano et al‚ 2012) and are probably the best characterized group of adhesion factors. The ALs proteins are in fact large glycoproteins that link to the β-1‚6 glucan in the cell wall of C. albicans. Als proteins all have a similar structure which
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Current status on: Biopharming: crops for the production of Therapeutic & Pharmaceutical proteins Biopharming‚ also known as molecular farming‚ is the production of pharmacologically active substances‚ either induced or increased through the application of genetic engineering. The first instance of artificial gene expression in an organism to produce a pharmaceutical product was the synthesis of insulin in the bacterium E. coli (Goeddel et al.‚ 1979). This type of biotechnology has since moved
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JAR 66 Module 9Exam Practice Exam Module 9 This is exam number 1. 1. Accidents and engineering faults are a) insignificant and increasing b)insignificant and decreasing c) significant and increasing Answer:c 2. Information on drugs and alcohol can be found in a) AWN 3 b)AWN 47 c) BCARs Answer:b 3. 70 - 80% of the total focusing ability of the eye is carried out by the a) iris b)lens c) cornea Answer:c 4. At what distance should a person without hearing difficulties be able
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