Brilliant Blue dye. Question 6: It is important to set up a blank to separate the solute (saline) from the protein (stock). By subtracting the absorbance of the blank (which has no protein present) from the original absorbance the absorbance of the protein at each concentration will remain. Question 7: The Lowry method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds‚ forming reduced copper in alkaline solutions. This is called a "Biuret" chromophore
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*‚ Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: September‚ 2010 ____________________________________________________________ _________________________________ Abstract: The experiment was done to separate and analyze the components of chili pepper. Chromatography was used because of its
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Being an exchange student has given me the chance to now spread out my opinions of a foreign country. And this may catch the attention of those who are also deciding upon taking the same sweet challenge‚ just as I did. I consider someone lucky when he or she gets the opportunity to live a fascinating experience one year abroad. I had the chance to live this experience in a little town called Horton‚ located in Alabama. An experience like this forces you to live so many different moments I was surrounded
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Reactivity of Halide Ions PURPOSE The purpose of this experiment is to observe the reactions of halide ions with different reagents by mixing them together. Materials 0.1 M AgNO3 0.1 M NaCl 0.1 M NaF 0.2 M KBr 0.2 M Na2S2O3 3% Starch Solution 4.0 M NH3 (aq) 5% NaClO (commercial bleach) 24 – well microplate thin – stemmed pipettes‚ 12 Safety DATA |PARTS |INITIAL MASS |FINAL MASS |CHANGE IN MASS | |
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Effect of ions on living organisms (24 marks) An ion is a charged atom or molecule. This is because it does not have an equal amount of protons and electrons‚ therefore giving the atom an overall charge. An example of an ion is ‚ this is sodium and the plus sign represents an overall positive charge. Ions are very important to living organisms as they are essential for life‚ they play an important role within the cell allowing mechanisms within the living organisms such as respiration‚ to take
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TOPIC: Role of students in the purification of the society. TABLE OF CONTENTS TOPIC: Role of students in the purification of the society * Purpose of education. * Students and their role in the society. * Current situation of the society. * Students’ reformers in the past. * Students’ role in the purification of the society. * Conclusion
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experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper‚ a tall clear glass‚ water‚ table salt‚ 1 tsp. measuring spoon‚ an 8 oz. measuring cup‚ pencil‚ ruler‚ 6 washable markers‚ and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown‚ teal‚ red‚ orange‚ black‚ and green. The salt solution served as the mobile phase while the chromatography paper served
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Chromatography of Food Dye Chem 180-DL3 Abstract: The goal of this experiment was to use different dyes to determine the Rf value. Chromatography is done to determine the presence of a mixture in a substance. In this experiment different dyes were used such as McCormick food coloring‚ M&M’s‚ Kool-Aid‚ and the dyes that came with the lab kit. Experiment and Observation: In this experiment a 50mL beaker was filled with luke warm water and a pinch of salt water was dissolved in it
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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