3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Medell’s Islamic Ancient Traditions: The Six Pillars Islamic Ancient Traditions: The Six Pillars From the Writings of Crystal Medell Humanities Ms. Melissa Jayne Shelton University of Central Oklahoma Abstract The purpose of this paper is to describe ancient Islamic traditions. This paper focuses on the religious traditions that are mainly still practiced today according to the Qur’an. The six pillars are described which consist of Shahadah‚ Salat‚ Zakat‚ Sawm‚ Hajj‚ and Jihad.
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Work Plan for Isolation‚ Purification‚ Identification and Starter Culture Activity of Lactoccocus lactis Submitted by: M.Usman Akram B.S. (Hons.) Dairy Technology mh.usman@hotmail.com Mobile : +923217773736 University of Veterinary and Animal Sciences‚ Ravi Campus Pattoki Lactoccocus lactis Classification: Scientific classification | Kingdom: | Bacteria | Division: | Firmicutes | Class: | Bacilli | Order: | Lactobacillales | Family: | Streptococcaceae | Genus:
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Water‚ Bread and Light are three important symbols in John’s Gospel. How do these symbols help the Johannine community to understand the identity of Jesus? The Gospel of John addresses the Johnannine audience’s journey of faith with theological reflections on the origin and identity of the “Word”. The author uses universal symbols of the light‚ the bread and the water to represent the identity of Jesus to the Johnannine community‚ thus reinforcing a high Christology. The signs performed by Jesus
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“We are punished for our refusals. Every impulse that we strive to strangle broods in the mind‚ and poisons us. The body sins once‚ and has done with its sin‚ for action is a mode of purification. . . . Resist it‚ and your soul grows sick with longing for the things it has forbidden to itself‚ with desire for what its monstrous laws have made monstrous and unlawful. (25).” In the beginning of the book‚ Lord Henry initiates a conversation about the act of sinning to Dorian. Lord Henry venturing out
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Fanaticism of Pure Race in the Early 1900’s Throughout history we have seen how humans must adapt or evolve to survive. But Germans took it to far by promulgating a purification of a race and tweaking the meaning of survival into races. In the early 1900’s the German society acted to convey the purification of their race called the German Volk‚ where women were persuaded to stay home and reproduce beautiful German children‚ suppress abortion‚ and sterilizing people from other races. The Nazis viewed
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Once the aspirin is prepared‚ it is isolated from the reaction solution and then it is purified. The aspirin is insoluble in cold water‚ and it is isolated by filtering the chilled reaction solution. Purification is essential to remove any unreacted salicylic acid and acetic anhydride as well as the acetic acid product and phosphoric acid. Acetic anhydride is caused to decompose by the addition of water once the formation of aspirin is complete. C4H6O3 (Acetic anhydride) + H2O (Water) ------------------------->
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Rhythm can rise and fall freely without losing underlying tension Character a large variety of characters can be used‚ each episode can involve new characters without needing to introduce them thoroughly. 2. Sobbing-a women sobbing 3. Purification-a women seeking purification 4. Nana story-grandchild 5. Photograph story- 6. Story of a father- women dealing with yet to come father death 7. Front and centre-kind stranger 8. Family gallery 9. Black skin girl 10. Invasion poem- 11. 1788- 12. Murri gets a dress-
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acetylsalicylic acid decomposing. The purpose of this procedure is to isolate acetylsalicylic acid from its accompanied acid catalyst‚ unreacted salicylic acid‚ acetic anhydride‚ and other impurities. Their removal can be done by crystallization‚ a purification technique in which‚ upon cooling‚ product crystals will be separated from impurities that are present in the solution‚ which can then be filtered off using a vacuum. In this procedure‚ acetic anhydride is used to wash the reactants‚ and phosphoric
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AIMS At the end of the experiment‚ students should be able to synthesise pure aspirin using chemical tests such as esterification and recrystallization independently and to also find out the melting point range of aspirin using melting point determination. INTRODUCTION Aspirin‚ which is also known as acetylsalicylic acid‚ is one of the commonly used and widely found in any drugstore or pharmacy. Its properties make aspirin a well-rounded drug that reduces inflammation and fever and also relieves
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