Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH nullifies the negative charge on the protein molecules. The pH of Buffer II is 4.7‚ which is < pI‚ the protein will be
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The purpose of this experiment was to synthesize triphenylmethanol from a Grignard reagent. The Grignard reaction technique was used in this synthesis but due to the fact that it is such a strong nucleophile and base‚ it was important to prevent water from interfering with the Grignard reaction. Purity of the product was determined by measuring the melting point. Reagent Table: Structure Name Molecular formula Molar mass Density Melting point Boiling Point Diethyl ether C4H10O
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solution would be put into the cuvette at precisely 30 seconds and so time would not have an effect on my results. I also used separate pipettes to measure out the starch‚ amylase and distilled water so that the solutions don’t mix together before the reaction should start. Also‚ I repeated each of my experiments two times to make sure I get accurate
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Organic Chemistry II Lab Diels Alder Reaction Purpose: In this experiment a Diels-Alder reaction was used to form the products. Cyclopentadiene and maleic anhydride were reacted together to form cis-Norbornene-5‚6-endo-dicarboxylic anhydride. 7-oxabicyclo{2.2.1}hept-5-ene-2‚3-dicarboxylic anhydride was also produced through a Diels-Alder reaction with the combination of furan and maleic anhydride. Equation: Procedure: Part 1 In a flask equipped with
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INTRODUCTION The lab introduced the relationship between reactants and products‚ and sought to discover which ratio of an acid and base reaction produced the most amount of carbon dioxide gas (CO2) without leaving leftover reactants. 5 varying amounts of bases were added to a constant amount of acid (10 ml) to better understand which ratio was the most efficient. RESULTS Data collected from the lab suggests that the ratio of acid to base that produced the most carbon dioxide gas (CO2) was 1:0.5
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ab: Observing a Chemical Reaction Name: Deja Eason Period: 5th Period Partners: Melingh Patterson‚ Tori Millens Date Performed: 9/2/11 Teacher: Mrs. Medina Abstract: This experiment was all about observing a chemical reaction. During the experiment‚ we made observations and possible explanations on why Copper(II)Chloride Dehydrate and Aluminum foil created a chemical reaction. The observations were made by using Copper Chloride‚ Aluminum foil‚ and Distilled water. Before the Aluminum
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________________________________ Date: __________________________ Lab Partner: _____________________________________________________________________ Investigating Factors that Affect the Rate of Enzyme Activity See Textbook Pg 61. Enzymes are very large complex organic molecules that are synthesized by the cell to perform specific functions. These biological catalysts are important because they speed up the rate of the reaction that would otherwise be too slow to support life. Catalase is
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Qualitative Observations of Double Displacement Reactions Lab Table 1.0 Qualitative Observation of Products Formed |Balanced Chemical Equations |Qualitative Observations | |BaCl2 (aq) + 2NaOH (aq)( BaOH2(aq) + 2NaCl(s) |An aqueous solution formed | | |Precipitate
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Chem 51LC Experiment 6 Lab Report Aldol Reaction Purpose: The purpose of this experiment is to be able to conduct an aldol condensation reaction using an unknown aldehyde and an unknown ketone. H NMR is used to identify the unknown aldehyde and ketone. Melting point is used to identify the aldol condensation reaction. Theory: Condensation reaction is also known as a dehydration reaction. In the mechanism of condensation reaction‚ a bond is formed between two molecules and creates water as a
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displacement reaction is a type of chemical reaction in which two compounds react in order to create two new chemicals. The two cations and anions switch places with each other which forms two new compounds. As an example AB+CC can switch to AD+CB. Both sides of these equations must balance out in order to be neutral. So any pairs of compounds (such as A+B) must have a total balanced charge. In this example A could have a charge of positive 2 (+2) and B could have a charge of negative 2 (-2) in order to
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