Determination of Gatifloxacin and Ornidazole in Tablet Dosage Forms by High-Performance Thin-Layer Chromatography 43 A simple and sensitive high-performance thin-layer chromatography method has been developed for the quantitative estimation of gatifloxacin and ornidazole in its combined dosage forms. Gatifloxacin and ornidazole were chromatographed on silica Gel 60 F254 TLC plate using n-butanol: methanol: ammonia (6 M) (8:1:1.5 v/v) as the mobile phase and scanned at 302 nm using a Camag TLC Scanner
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Objective: To determine the concentration and equilibrium constant for a reaction of colored product using absorption spectroscopy‚ to create a calibration curve for determining equilibrium concentrations by applying Beer’s law and to force the completion of a reaction by applying Le Châtelier’s principle. Procedure: A diluted solution was made from the stock of 0.0025 M Fe(NO3)3 by diluting 4.0 mL to exactly 100.00 mL‚ using serological pipet and volumetric flask. The concentration of the diluted
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http://www.www.ijpsonline.com on Friday‚ July‚ 4 2008] www.ijpsonline.com Short Communications Spectrophotometric Method for Determination of Tablets Glucosamine in Tablets PRIYA GAONKAR‚ VINEETA KHANVILKAR‚ RAJANI SHETTIGAR AND CHHAYA GADGOLI* Saraswathi Vidya Bhavan’s College of Pharmacy‚ Sonarpada‚ Dombivli (East)-421 201‚ India. A rapid and sensitive method has been developed for the determination of glucosamine sulphate from tablets by UV-spectrophotometery. In this method‚ glucosamine
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was found by titrating a series of reactions containing H2O‚ HCl‚ and the unknown ester with only the last solution containing the unknown alcohol. Equilibrium constants can be easily found once the concentrations of all reactants are known using Keq= [ acid][alcohol]/[ester][water]. Procedure: Part A: Prepare six bottles with the following volumes in each: Bottle # 3M HCl in mL H2O in mL Ester in mL Alcohol in mL 1 5 5 0 0 1/A 5 5 0 0 2 5 0 5 0 3 5 1 4 0 4 5 3 2 0 5 5 2 2 1 Once the
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cytosol(Nelson and Cox 2008). To investigate these reactions we measure the amount of urea produced with the use a sample of liver extract containing arginase. This is then added to solutions of arginine and water. We use colorimetric determination and spectrophotometric analysis as a result of the red colour produced by the solution’s reaction with α-isonitrosopropiophenone (Davis and Mora 1968). Manganese sulphate is added to the solutions as the outer manganese ions in reaction recovered the activity
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Observations | 1 | no colour change‚ less precipitate | 2 | turned light blue‚ less precipitate | 3 | turned grey‚ | 4 | no precipitate | 5 | turned dark blue‚ less precipitate | 6 | more precipitate | 7 | turned light blue | The Keq expressions for the reactions in test tubes 1-5 were determined. There was no test tube where
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Experiment 11 Title: Spectrophotometric analysis of caffeine and benzoic acid in soft drink. Name: Tan Herh Lim Name of partner: Sia Ting Wai‚ Chong Zheng Yee Date: Lecturer: Dr. Neo Kian Eang Practical class: P1 Objective: To obtain the absorbance of the caffeine and benzoic acid in soft drinks. Introduction Soft drinks that commonly found in our daily life contain caffeine and sodium benzoate. The caffeine act as a stimulant and the sodium benzoate acts as a preservative
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NOTES AND COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error
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reaction used for calibration. 3. What is the heat capacity of the calorimeter? Relate its sign to the sign of the ΔT. 4. In the appendix‚ show the derivation to obtain the equation used to calculate the heat capacity of the calorimeter. Determination of Heats of Reaction: 5. Give the net ionic equation for each reaction. 6. Determine the limiting reactant and the amount of the limiting reactant in each of the reaction performed. 7. Calculate for the theoretical and experimental enthalpy
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Experiment 3 Spectrophotometric Analysis of Mixtures: Simultaneous determination of two dyes in solution Reading: Appendix J Harris‚ Section 2.5‚ Chapter 18‚ and Section 19.1 Introduction Spectrophotometric determinations based on the Beer-Lambert Law are among the most widely used analytical procedures. These methods involve the measurement of the fraction of incident electromagnetic radiation that is absorbed by a sample. To determine the concentration of a colored species in solution
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