EXPERIMENT 2 ACID DISSOCIATION CONSTANT OF AN INDICATOR DYE OBJECTIVES Using spectrophotometric method: determine the wavelengths at which the acid and base forms of the dye in aqueous medium exhibit maximum absorption; determine the molar absorptivities of the acid and base forms of the dye and estimate an unknown concentration of the dye in solution using the Beer-Lambert’s Law; and determine the acid dissociation constant of the indicator dye. THEORY The absorption or reflection of
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Determination of Sugars in Sports Drinks: A Spectrophotometric Analysis By: Crystle Culling Student Number: 2686923 Class: Tuesday 12pm (odd weeks) Page | 1 1015MSC Lab Report Crystle Culling 2686923 Abstract The concentration of sugars in two well-known sports drinks‚ Powerade and Gatorade‚ were determined by monitoring an enzyme-catalysed reaction sequence involving the appearance of NADPH. Sucrose and glucose concentrations were calculated from the concentration of NADPH formed by the reaction
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The Spectrophotometric Analysis of Copper in a Copper Oxide Ore Abstract In this experiment‚ the mass percent of copper in an unknown copper oxide ore was determined by using a spectrophotometer and Beer’s Law. The mass percent of copper was found to be 5.12 0.14%. Introduction In spectrophotometric analysis‚ the concentration of a solute is measured in a solution by measuring the amount of light that is absorbed by the solution in a cuvette placed in a spectrophotometer. A spectrophotometer
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contingency of the temperature value fluctuating due to environmental influences. REFERENCES [1] http://chemwiki.ucdavis.edu/Physical_Chemistry/Spectroscopy/Electronic_Spectroscopy/Electronic_Spectroscopy_Basics/The_Beer-Lambert_Law [2] Beer.1852. Determination of the absorption of red light in colored liquids. Annalen der Physik und Chemie‚ vol. 86‚ pp.
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1.1: Thesis outline Purines breakdown by the body will form a chemical called uric acid. Uric acid mostly can be found in foods and drinks like dried been and pears. Normally‚ most of the uric acid dissolves in blood and move from blood to the kidneys. High level uric acid kidney or blood caused some bad effect such as leukemia‚ hyperuricemic and lymphoma. Therefore‚ the electrochemical method (graphene oxide modified glassy carbon electrode method) are introduced to detect the uric acid in human
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Chemistry 12 Unit 2 Notes - Equilibrium Chemistry 12 Tutorial 5 – The Equilibrium Constant (Keq) What is Keq ? The "K" in Keq stands for "Constant". The "eq" means that the reaction is at equilibrium. Very roughly‚ Keq tells you the ratio of Products/Reactants for a given reaction at equilibrium at a certain temperature. [Products] [Reactants] K eq = It’s not quite this simple when we deal with real substances. Let’s take an example. It has been found for the reaction: 2HI(g )
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bB ⇌ cC + dD kf[A]a[B]b = kr[C]c[D]d kfkr = Keq = [C]c[D]d (1) [A]a[B]b A factor in determining whether to which direction a reaction will go to that has not yet reached equilibrium‚ is the reaction quotient Q. Q is just the same as the Keq expression‚ but the main difference is that the concentrations of the reactants and the products used in the equation are still not yet at equilibrium. Whenever Q<Keq‚
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direction of the reaction 4. What does a K value represent for an equilibrium reaction? Write the generic equation for K 5. Match the following K values to the graphs that you created in question 3: a. Keq = very large b. Keq = 1.0 (approx.) c. Keq = 5.0 x 10-12 d. Keq = 3.5 x 102 e. Keq = very small 6. Outline Le Chatalier’s principles of equilibrium with respect to addition or removal of reactants and products‚ change in temperature for exo and endothermic reactions‚ and change in pressure
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in distilled water: Cu(OH)2(s) (( Cu2+(aq) + 2OH-(aq) Ksp Cu(OH)2 Keq = Ksp Cu(OH)2 = 2.2 x 10-20 b. + 6.0M H2C2O4 Cu(OH)2(s) (( Cu2+(aq) + 2OH-(aq) Ksp Cu(OH)2 H2C2O4 (( H+ + HC2O4- Ka1 H2C2O4 HC2O4- (( H+ + C2O42- Ka2 H2C2O4 Cu2+ + C2O42- (( CuC2O4 1/Ksp CuC2O4 2(OH- + H+ (( H2O) 1/K2w Net Keq = (Ksp Cu(OH)2 x Ka1 x Ka2 )/Ksp CuC2O4 = 1.969 x 10-2 c. pinch
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ANALYTICAL BIOCHEMISTRY Analytical Biochemistry 334 (2004) 196–198 www.elsevier.com/locate/yabio Notes & Tips A spectrophotometric assay for the quantiWcation of polyethylenimine in DNA nanoparticles Martin Bertschinger‚ Sophie Chaboche‚ Martin Jordan¤‚ Florian M. Wurm Swiss Federal Institute of Technology Lausanne‚ Laboratory of Cellular Biotechnology‚ Lausanne VD1015‚ Switzerland Received 29 April 2004 Available online 28 August 2004 Since the Wrst description of polyethylenimine (PEI)1 as a
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