Lab Two; Chromatography of Food Dyes Jan Konig 28.04.2013 11:00am Chemistry 121‚ section DE Abstract: The second lab deals with the chromatography of food dyes which means you figure out what kind of compounds are used in a product like food color etc. by comparing the result to FD&C Food Dyes. You use six different food dyes‚ four food colors out of the store‚ two different Kool Aid powder and four different colors of candy like M&Ms you use for the chromatograph. At the end you try
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Name: (e.g.‚ BioLab 3.4_A_Jones.doc) MDean Date: Graded Assignment Lab Report Answer the questions. When you are finished‚ submit this assignment to your teacher by the due date for full credit. (5 points) 1. Describe generally what happened to each spot of each type of ink. Which had the most pigments? Answer: The black ink went from a light blue to dark blue then to red. The red ink went from red to pink. It went the furthest out of the inks. The green ink went from dark blue to green and then
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COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos‚ Kuia B. Allarde‚ Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment‚ the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
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Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
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Column and Thin layer Chromatography of Red Siling Labuyo Marian Angelu Ramos‚ Rose Ann Refuela‚ Leomarie Duanne Sanchez‚ Paula Lynne Santos‚ Geraline Sarmiento‚ Jon Carlo Semana Group number Seven‚ 2E- Pharmacy‚ Faculty of Pharmacy‚ University of Santo Tomas ABSTRACT Chromatography is the separation of mixtures into their constituents. It relies on the differential solubilities or absorptivities of the components to be separated with respect to two phases‚ one of which is stationary and the
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possible to identify which peak correlates with which compound. Figure 2 contains a peak at around 500 and 700 nm which is a good indicator that that would-be chlorophyll b. This makes sense because this was the bottom band from the thin layer chromatography and chlorophyll b is the most polar compound. Chlorophyll B contains an aldehyde where chlorophyll A has a methyl group making it slightly more polar. Following that‚ figure 4 contains
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Chromatography (Greek for ‘colour writing’) is used to describe various methods applied to separate mixtures (referred to as the sample of the experiment) with great accuracy to analyze them. By using chromatography we can manipulate these to move at different speeds through the system‚ thus separating them. Chromatography is necessary in chemical industries‚ as well as bio processing companies. Chromatography can be: 1. analytical: used to measure ratios of analytes(substance in simpler forms)
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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