...................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ............................................................ 25 1. Chromatography ................................................................... 25 2. Electrophoresis ..................................................................... 53 III. Immunoassays .............................................................
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Performance Liquid Chromatography but is also referred to as High Pressure Liquid Chromatography. It is used for separating mixtures either to analyse the mixture or to separate a required product from others in a reaction mixture. It can also be used to find the relative amounts of different components in a mixture. HPLC works along the same lines as paper chromatography. In paper chromatography a liquid (mobile phase) moves past a solid (stationary phase). In paper chromatography the stationary phase
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To make a statement of the problem in investigatory projects‚ come up with a detailed paragraph outlining the essential issue that you will be investigating. If your teacher wants a hypothesis‚ or educated guess‚ consider the problem‚ outline it in a paragraph‚ and then add your educated guess about what will happen in your investigation. The best hypothesis will be the result of research and logic. Once you’ve decided how to describe a problem in four sentences or so (use more paragraphs if needed)
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silica gel‚ which acts as a Lewis acid catalyst to facilitate the reaction. The reaction forms a porphyrinogen‚ which is then oxidized to the porphyrin product by atmospheric oxygen. Column chromatography is performed for the isolation and purification of tetraphenlyporphin‚ and the thin layer chromatography for analysis.It was concluded that the renention factor(Rf) of the 5‚10‚15‚20- Tetraphenylporphyrin with a percent yield of 61% Introduction: In this experiment 5‚10‚15‚20‚ tetraphenylporphyrin
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Humans always have the thought of healthy in their head‚ but not all are the same. The real way to eat and stay healthy is to eat superfoods and for example‚ a wrap containing avocado‚ smoked salmon‚ beans‚ and peppers wrapped in a healthy spinach tortilla. These all contain the needs for a meal to support a human and is also very healthy. To start‚ I chose all these items because in all‚ a person needs carbohydrates‚ protein‚ fats‚ vitamins‚ minerals and water to survive. So‚ with all these components
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acid-catalyzed dehydration of 1-butanol and 2-butanol using sulfuric acid and a dehydrobromination under basic conditions using potassium tert-butoxide of 1-bromobutane and 2-bromobutane. The products of all four reactions will be analyzed with gas chromatography‚ which separates organic compounds to see how each reaction’s product are formed under the constraints of stereochemistry and regiochemistry. In a general elimination reaction‚ an atom or group of atoms is removed from a carbon‚ along with an
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METHODS FOR FUNCTIONAL FOODS & NUTRACEUTICALS LABORATORY REPORT LAB 2: FAT & OIL FROM OIL-SEEDS: PART II THIN LAYER CHROMATOGRAPHY ANALYSIS (TLC) COORDINATORS: ASSOC. PROF. DR. JAMALUDDIN BIN MOHD DAUD ABDUL AZIM ADNAN 1016741 INTRODUCTION TLC chromatography or thin layer chromatography is a type of planar chromatography. TLC is routinely used by researcher in the field of phyto-chemicals‚ biochemistry etc. to identify the components in a compound mixture
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Chemistry Lab Write-Up Title: Paper Chromatography of Powdered Beverage Mix Purpose/Objective: The purpose is to be able to use liquid chromatography to separate dyes in a powdered beverage mix‚ calculate the Rf values for each dye‚ and rank the polarity of each dye present. Hypothesis: If we are to use the liquid chromatography to investigate the different dyes‚ then I think the different dyes will come up having different polarities and Rf values. Procedure/Method: Draw a line across
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the retention time of polar molecules is shorter because they pass through the column faster. Reversed phase HPLC is the most commonly used form of HPLC which separate solutes based on non-polar differences. In this experiment‚ reverse phase chromatography was used. The more polar antioxidant is expected to elute first due to the weak attraction to the non-polar column. Figure 2 below shows the structures of the two antioxidants. Both antioxidants contain a benzene ring and a hydroxyl group. However
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BHTM 370 – SANITATION AND HEALTH IN FOOD SERVICE Instructor: Accaoui Antoine Name: Sarah khalifeh ID: 31030483 Section: A Subject: Recipe of Lasagna‚ Polo Alfredo and Chicken Caesar‚ and HACCP chart from storing to serving Recipe of Lasagna: Ingredients: * 1 pound sweet Italian sausage * 3/4 pound lean ground beef * 1/2 cup minced onion * 2 cloves garlic‚ crushed * 1 (28 ounce) can crushed tomatoes * 2 (6 ounce) cans tomato paste * 2 (6.5 ounce) cans
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