The plant material (10 g) used‚ was a representative sample of C. sativus stigmas obtained from the Cooperative De Safran (Krokos Kozani‚ Greece) in 2012. The ISO 3632-1 (ISO 2011) quality parameters E1%1cm calculated at 440 nm‚ 257 nm‚ and 330 nm for an aqueous extract were 253‚ 86 and 34 respectively‚ indicating that the plant material was of the highest quality (Category I). 2.2. Standards‚ reagents and solvents Safranal (>88%)‚ isophorone (>97%)‚ keto-isophorone (>98%) and 2-phenylethanol (>99%)
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protein content was estimated and the concentration was adjusted to ~40 µg. Concentrated lectin in the leaf sample was separated by SDS-PAGE (12% gel) according to Laemmli 1970‚ the fractionated proteins were transferred onto PVDF membrane (0.45mm; Millipore USA). After blocking for nonspecific binding using 3% BSA‚ the membrane was incubated with rabbit anti-SRL polyclonal antibodies at 1:5‚000 dilutions followed by treatment with a secondary antibody‚ horse radish peroxidase (HP) conjugated anti-rabbit
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purchased from Aldrich (St. Louis‚ MO‚ USA). Acetone was purchased from Fischer Scientific Laboratory (Fair Lawn‚ NJ‚ USA). Phosphate buffer pH 7.0 was purchased from EMD Chemicals Inc. (Gibbstown‚ NJ‚ USA). 0.2 micron syringe filters were obtained from Millipore Corporation (Carrigtwohill‚ Ireland). 1.1. Collection and preparation of crude methanolic plant extract (CMPE) of Callistemon citrinus (CC) Sample collection: The plant used in current study was Callistemon citrinus and it was collected from various
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INTRODUCTION An important aspect of any biotechnological processes is the culture of animal cells in artificial media. These animal cells in culture are used in recombinant DNA technology‚ genetic manipulations and in a variety of industrial processes. Now-a -days it has become possible to use the cell and tissue culture in the areas of research which have a potential for economic value and commercialization. The animal cell cultures are being extensively used in production of vaccines‚ monoclonal
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Minor Project 1: Cochlear vs Proteome Systems Limited by Yan He‚ Li Fan‚ Tamara Leahy‚ Jo Whittaker 1. Introduction Intellectual property has historically been regarded behind raw materials and capital in importance‚ but is increasingly being regard as the most important aspect of business success [1]. Companies that recognise the importance of intellectual property‚ and manage it accordingly‚ are better positioned to receive economic benefits[2]. However‚ in the biotechnology industry
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A cross-industry review of B2B critical success factors Riyad Eid Myfanwy Trueman and Abdel Moneim Ahmed Introduction In recent years business-to-business international Internet marketing (B2B IIM) has received widespread attention. Avlonitis and Karayanni (2000)‚ Hamill and Gregory (1997)‚ Hoffman et al. (1999)‚ Porter (2001) and Quelch and Klein (1996) conducted in-depth studies to understand those factors that are needed to enhance B2B IIM implementation. Various articles‚ empirical research
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Pakistan J. Zool.‚ vol. 43 (5)‚ pp. 849-854‚ 2011. Comparative Efficacy of Various Antiviral Agents against Avian Influenza Virus (Type H7N3/Pakistan/2003) Tahir Mahmood Shaukat‚1 Muhammad Ashraf‚ 1 Muhammad Ovais Omer‚ 1 Muhammad Adil Rasheed‚ 1 Khushi Muhammad‚ 2 Tariq Mahmood Shaukat‚ 1 Muhammad Younus3 and Muhammad Khurram Shahzad4* 1 Department of Pharmacology and Toxicology‚ University of Veterinary and Animal Sciences‚ Lahore‚ Pakistan 2 Department of Microbiology‚ University of
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Questionnaire to assess the importance of each competency in the overall performance of the job at Top Level Please rate the importance of each competency using the 5-point rating scale This competency is of vital importance to a successful performance of my job | 5 | This competency is definitely important to the successful performance of my job | 4 | This competency is relevant but not important to the successful performance of my job | 3 | This competency is of highly marginal relevance
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solution. The reaction mixture was maintained basic medium using 1 g of NaOH solution was added drop by drop into the above metal solution. The mixture was stirred for 30 min and the precipitate was poured into the 50 ml auto clave vessel using Millipore water. The solution was aged homogeneously at 160 oC for 12h. After that the solutions was centrifuged at 8000 rpm and washed many times using DD water with ethanol. The final products are dried at 60 oC in hot
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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