The Effects of pH‚ Temperature‚ Enzyme‚ and Substrate Concentrations on Benzoquinone Production BIOL 2051 June 10th 2013 Introduction Enzymes are the ultimate catalysts of living things. Enzymes are made of proteins which are structured and directed by amino acids chains. Enzymes attract and fit substrate molecules to an active site. The active site binds the substrate molecules covalently to enzyme forming an enzyme-substrate complex‚ which catalyzes
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higher concentration to those of lower concentration‚ until there is an even concentration. This movement is random because it is a result of kinetic energy. Diffusion is a slow process‚ but there are many factors that will affect it’s rate. The rate of diffusion will vary depending on the size of the particle. For example‚ a smaller particle will move faster than a large molecule at a given temperature . During reactions‚ when there is more energy‚ the reaction will take place at a faster rate. An
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The Effect of Concentration Gradient on Osmosis Abstract: Osmosis is the passive movement of water from an area of higher concentration to an area of lower concentration‚ usually across a membrane (Thorpe 2013). Tonicity is the ability of a solution surrounding a cell to gain or lose water (Reece 2011). There are many factors that affect the rate of osmosis. These include temperature‚ surface area‚ difference in water potential‚ pressure‚ light and dark and most importantly what we will be talking
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Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
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20th‚ 2012 Enzyme Lab What is an enzyme? Enzymes are specialized protein molecules simplifying most of the body’s metabolic processes such as‚ supplying energy‚ digesting foods‚ purifying your blood‚ executing the body of waste products etc. Enzymes act as catalyst by speeding up the reactions that happen in our bodies and decreasing the amount of activation energy needed to break a complex down. A reactant is any given enzymatic reaction is called a substrate for that specific enzyme. The place
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Enzymes play a vital role in helping our body function. They act as biological catalysts and help speed up reactions that would otherwise take long periods of time to naturally occur. Enzymes help lower the activation energy required for the reactants to reach the transitional state from which then they can form products. However‚ enzymes do not change the free energy of the reaction. Enzyme’s ability to catalyze reactions comes from the shape of the active site on the enzyme. Enzymes are hyper-specific
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Chemical reactions proceeding until all the reactants are used is a common misconception. Chemical reactions actually behave differently. The general reaction equation is a A + b B → c C +d D in this equation A and B are the reactants forming the products C and D. However‚ unlike the common thought that the reaction ends when it runs out of A and B it actually does not. In most reactions C and D start to react to form A and B at a certain point as you can see in the equation c C + d D → a A + b
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Introduction How does changing the surroundings of enzymes affect their reaction rate? The purpose of the experiment is to determine how different abiotic conditions affect the rate at which enzymes accelerate/cause reactions In this lab students measured the height of the foam after catalysis between catalase (enzyme) and 7 other (solutions) to determine which solution had the fastest reaction rate.. The control variable of the experiment would be the solution of only hydrogen peroxide‚ water
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expressions is the Henderson-Hasselbalch equation? (Points : 10) pKa = pH + log pH = pKa + log pH = pKa + log pKa = pH - log pH = pKa + log 4. The Ksp of CaSO4 is 4.9 10-5 at 25 C. What is the concentration of Ca2+ in a saturated solution of CaSO4? (Points : 10) 2.4 10-9 M 2.5 10-5 M 4.9 10-5 M 7.0 10-3 M 3.7 10-2 M 5. What is the pH of the buffer that results when 12.5 g of NaH2PO4 and 22
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Determining Order from Rates of Reactions AP Chemistry Purpose The focus of this experiment is to recognize that when aqueous solutions of potassium iodate ion (KIO3-) and bisulfite ion (HSO3-) are mixed‚ a series of reactions will occur‚ and the final reaction is signaled by the appearance of a dark blue color. My partners and I investigate how the concentration of the reactants affects the rate of reaction. The purpose of this lab is to find
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