1. Analyse the operation in term of the Servuction model (spilt the operation into as many or few components as you like). What opportunities exist for separation between the visible and invisible service delivery components allowing for streamlining of the “factory”? Hoffman (2011) claims‚ “Service knowledge is acquired differently‚ than knowledge pertaining to goods”. For example: Due to the intangible nature of service it cannot be sampled before‚ but can only be experienced through simultaneous
Premium Customer service Good Customer
Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
Premium Chromatography Analytical chemistry Silica gel
Problem: Why do leaves change color in the fall? Background: Leaves contain many pigments such as chlorophyll b‚ xanthophyll‚ and beta carotene besides chlorophyll a. They are part of the photosystem in the thylakoids. Pigment molecules can be separated by a procedure called chromatography. The procedure uses a special paper and solvent. The chlorophyll molecules adhere to the paper. The solvent molecules move up the paper by capillary action. Each chlorophyll molecule will travel up the paper at
Premium Chlorophyll Photosynthesis
Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent material in the column separated the different pigments by allowing the least
Free Solvent Acetic acid Ethanol
Lab PART A. Separation of Plant pigments Access the following website: http://www.phschool.com/science/biology_place/labbench/lab4/intro.html (you will need to "cut and paste" this into your browser) You should see the Plant Pigments & Photosynthesis lab. Under the figure of the notebook‚ click the "next" button. Read "Key Concepts I: Plant Chromatography" Then click "next" and read "Design of Experiment I" Then click "a closer look". Read "Depositing the Pigment"‚ then click "next"
Premium Photosynthesis Color Light
using Chromatography and a Spectrophotometer In this experiment‚ the separation of particular pigments‚ contained within a fresh spinach leaf‚ were examined. Paper chromatography is the process of separating certain molecules‚ or pigment molecules‚ based upon their polarity. Four different pigments were examined from the spinach leaf: chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and carotene. Based upon looking at the pigments individual molecule structures‚ our group hypothesized that the most polar
Premium Visible spectrum Electromagnetic radiation Light
the silica. More eluent was added and drained until it was level with the top of the sand. Extraction of pigments 3-4 baby spinach leaves were gathered and placed into a mortar with a small amount of sand. 8mL of methanol was measured out and added to the mortar. A stemmed funnel lined with a filter was clamped and placed into a 125mL separatory funnel (with stopcock closed). The spinach was crushed with a pestle until it turned a dark green
Premium Water Oxygen Acetic acid
Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
Premium Chromatography Analytical chemistry Silica gel
Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
Premium Photosynthesis Color Green
separate plant pigments using paper chromatography‚ and to measure the rate of photosynthesis in isolated chloroplasts. Because of capillary action the solvent moves up the paper causing the pigments to become visible at certain distances. The substances visible on the paper are called pigments. Chlorophyll a is the main pigment that makes up about 75% of the pigmentation in plants. Chlorophyll b makes up about 25% of the pigmentation. And carotenes and xanthophyll’s are accessory pigments that make up
Free Photosynthesis Chlorophyll