Mr. Sousa Organic Chemistry ACL 8 January 2015 Chromatography Chromatography is a physical method of separating substances based on their properties‚ by distributing their components between a mobile and stationary phase. Chromatography is useful for observing mixtures and solvents‚ since it can be used to determine the relative bond strength of various compounds‚ a substances phase‚ and it can also the identity of unknown substances. Chromatography allows for the separation of chemical mixtures
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Abstract: The optical and photocatalytic properties of Nb2O5 sculptured thin films (STF) are investigated. Films are synthesized via sol- gel route using spin coating technique. The sol is prepared from niobium ethoxide. The films are prepared at different rpm to tailor the thickness. The structures of the films are investigated through XRD measurements. Refractive index‚ extinction coefficient and thickness of the films are determined from Variable Angle Spectroscopic Ellipsometery. The absorbance
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Bleach Oxidation of 9-Hydroxyfluorene The purpose of this experiment was to oxidize an alcohol (9-hydroxyfluorene) to a ketone (9-fluorenone) using aqueous sodium hypochlorite (bleach) as the oxidizing agent‚ while introducing techniques used in microscale experiments. Reaction: Results 1. Recrystallized Product Yield Product yield = (actual yield/theoretical yield) x 100% 3mL 9-hydroxyfluorene x (1mL/1000mL) x (0.09 mol/L) = 2.7 x 10-4 moles 0.05g 9-fluorenone / (180.20g/mol) = 2.77 x 10-4
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of the pure hexane and one mL of the pure acetone to the mortar. Grind the contents until the cell walls have been adequately broken. Transfer the mixture to a centrifuge tube to separate the sample into layers‚ to help remove the water. Once the sample is finished spinning extract the organic layer and place in a test tube. TLC: To determine which mixture acetone and hexane is the best developing solvent the first step is to make mixtures of these solvents in test tubes. In the first test tube
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provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1 2 3 4 ____________________________________________________________ ________________ b) Give examples of mixtures which can be separated through chromatography. 4 5 6 7 ________________________________________ ______________________________________ c) How is chromatography done? 4 5 6 7 ________
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Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
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Serratiopeptidase drug was purchased from MARS Therapeutics & Chemicals limited‚ Hyderabad‚ India. The standard drug Fenofibrate was purchased from USV limited‚ Himachal Pradesh‚ India. Assay kits for serum Total Cholesterol (TC)‚ Triglycerides (TGL)‚ High density lipoproteins were purchased from Erba Mannheim‚ Transasia Bio-Medicals limited‚ Himachal Pradesh‚ India. All other chemicals were of analytical grade. The experiment was conducted using [12] male Albino Wistar rats (150-200g)‚ at about
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Experiment 1: TLC Analysis of Analgesic Drugs 1/23/2011 Purpose: The goal of this experiment is to test our knowledge and understanding of TLC analysis by having us do a TLC analysis of analgesics to figure out their main chemical components. Calculations: 1.) Rf = Distance spot traveled/ distance solvent traveled Results: Table 1: TLC Analysis Analgesic Drugs | Rf Value | Acetaminophen | 0.323 | Aspirin | 0.597 | Caffeine | 0.081 | Unknown 154 (Plate 1) | 0.081‚ 0.306‚ 0.597
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3.6. Conclusion Various solvent extracts from mahua flower and fruits showed varying degrees of antioxidant activity in different test systems in a dose-dependent manner. The antioxidant activity was correlated with the amount of total phenolics present in the respective extracts in each assay. 50% acetone proved to be the most efficient solvent for extraction of antioxidants from mahua as the related extract contained the highest amount of phenolic compounds and also exhibited the strongest antioxidant
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As we know‚ the TLC is a separation technique that has two phases: the stationary phase and mobile phase. The stationary phase is silica gel (SiO2) which is very polar compound‚ while the mobile phase is 0.5% acetic acid which has less polar. Also‚ as we know‚ the compound will rather to stay with stationary phase or travel with mobile phase depend on its polarity. The result show as that the polarity of the solvent is the key factor for the compound to travel with it or stay in stationary phase
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