How Temperature Affects Amylase Activity Abstract This study of the effect of temperature on the enzyme amylase was performed to determine the relationship between the enzyme amylase and temperature. The rate of reaction was found to increase as the temperature of the environment was raised. As the temperature was raised from 5°C‚ 20°C‚ 35°C and finally to 80°C the rate of reaction followed this trend and also increased. However as predicted in the hypothesis of this experiment when the temperature
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Immobilized Enzyme Immobilized Enzyme * Enzymes which are attached to small bead made of alginate are called immobilized enzyme * However‚ whatever the nature of an immobilized enzyme‚ it must comprise two essential functions‚ namely the non-catalytic functions (NCF) that are designed to aid separation and the catalytic functions (CF) that are designed to convert the target compounds within the time and space desired * Immobilized enzymes might meet the increasing demand by manufacturers
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most vitamin C to the point where it will change colors. HYPOTHESIS: Orange juices labeled A will have the most enzyme concentration because it looked thicker than the others. Therefore I thought it will need more Iodine whilst orange juice labeled B & C will need less Iodine because they looked lighter. Variables Independent: the amount of Iodine that was added to the the different types of orange juice Dependent: The color of the orange juice changed to dark purple depending on how much iodine
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[List 3-digit number] Meeting Time: [Fill in the lab meeting time] TA: [Fill in the lab TA’s name] INTRODUCTION In this experiment I calculated the equilibrium constant for the reaction shown below under different conditions to determine if the equilibrium constant is really constant. Fe3+ (aq) + SCN– (aq) ←→ FeSCN2+ (aq) A big part of this lab includes understanding (and in the end proving) that absorbance is directly proportional to the concentration of a solution (Beer’s Law)‚ and
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Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the experiment
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reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three
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VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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Enzymes and Their Importance in Plants and Animals Enzymes are large proteins that are responsible for catalysing thousands of metabolic processes that sustain life. Enzymes work similarly to those catalysts used in industry by lowering activation energy and therefore dramatically speeding up the rate of a reaction‚ however‚ these biological catalysts are highly selective to their substrate. Almost every chemical reaction that occurs in a cell requires enzymes in order to occur at rates required
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the highest
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of temperature on fungal amylase and bacterial amylase (Aspergillus oryzae and Bascillus Licheniformis). We used 4 different temperatures in Celsius 0‚ 23‚ 58‚ and 89 for both fungal and amylase. For 10 minutes‚ every 2 minutes we would use 3 drops of each amylase and mix it with iodine to observe the presence of starch at each temperature. We conducted this experiment for both bacterial and fungal amylase. Results were reached based on the color of the iodine and amylase mixture. Colors were classified
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