Skoog/Holler/Crouch Principles of Instrumental Analysis‚ 6th ed. Chapter 14 Instructor’s Manual CHAPTER 14 14-1. Letting the subscript x stand for the unknown solution‚ x + s stand for the unknown plus standard‚ and Vt the total volume of solution‚ we can write Ax = ε bcxVx / Vt Ax + s = ε b(cxVx + csVs ) / Vt Dividing the first equation by the second and rearranging gives cx = Ax csVs 0.656 × 25.7 ×10.0 = = 21.1 ppm ( Ax + s − Ax )Vx (0.976 − 0.656) × 25.0 14-2. Using the equation
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As stated previously the purpose of this experiment was primarily based on about chemical changes. Many chemical changes are viewed through a screen‚ or read from a book. In this experiment‚ chemical changes are viewed directly from the eyes of the scientist. The procedure first begins by gathering all needed materials. Next‚ laying the first powder on four wells‚ followed by the next powders‚ each powder was placed on four wells. The liquids were then placed on each powder using the four droppers
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In labs 4.1‚ 4.2‚ and 4.3‚ the connection between the force of gravity and the type of surface was tested through a series of experiments. In lab 4.1‚ the force of gravity was tested through various surface types‚ including: tabletop‚ waxed paper‚ paper towel‚ fine sandpaper‚ and coarse sand paper. In lab 4.2‚ two different surface types that were tested included: tabletop and coarse sand paper. This lab compared the two averages between both tabletop and coarse sandpaper using different amounts
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Alcohol Lab Data Conclusion Objective: The objective of this lab was to find the concentration of an unknown alcohol solution. Procedure and Data: We found the masses of 10.00 mL of each of the known concentrations of alcohol (minus the beaker mass) by placing the beaker onto the scale‚ zeroing the scale‚ and then pouring alcohol into the beaker‚ repeating for each different concentration solution. Then‚ using the same method‚ we found the mass of 10.00 mL of the unknown concentration. We recorded
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The lab today was focused on finding the ratio of reactants to products to be either 1 to 1 or 1 to 2. In our case the reactants was Lead (II) Nitrate and Potassium Iodine. These two when mixed together make Lead Iodide and Potassium Nitrate. We also had to try and find if the number of moles of Lead(II) Nitrate was the same as the final number of moles for Lead Iodine after the experiment. Our data for the lab had pinpoint accuracy. Proved by the data table below Trials Volume of Pb(NO3)2 Mol
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Direct competitive cocaine immunoassay was performed in a transparent high binding 96 well microtiter plate. Each well was coated with 200 µL of 1mg/L anti-mouse IgG in PBS (phosphate buffered saline‚ 10 mM sodium dihydrogen phosphate‚ 70 mM sodium hydrogen phosphate‚ 145 mM sodium chloride‚ pH 7.6). The microtiter plate was left over night for shaking at 750 rpm. Therefore‚ the plate was washed three times with automatic 96-channel plate washer by washing buffer (45 M potassium dihydrogen orthophosphate
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METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
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The two spots obtained from tube 1 are glutamate and the product of pyruvate which is alanine. The only spot from tube 2 is glutamate. The 2 spots obtained from tube 3 are alanine and the product of α-ketoglutarate which is glutamate. Moreover‚ the only spot from tube 4 is alanine. Tube 5 is a control group. Therefore‚ no reactions are occurred‚ and no spots on the paper. The reaction for tube 2&4 are different from other tubes is because the enzyme transaminases that stayed in the tubes get denatured
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An investigation into the total acid content of assorted brands of lemon and lime soft drink Abstract: The aim of this experiment was to determine the total levels of carbonic acid concentration in 3 differing brands of lemon and lime soft drinks. Research Question: Does the acid content in three different lemon and lime flavoured soft drink brand differ significantly? The purpose of this investigation is to calculate the total acid content within different brands of lemon
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William Piumbroeck Chem 214 Acid-Base Titration‚ Determination of Carbonate and Bicarbonate in a water sample Introduction The purpose of this lab is to determine the concentration of two bases‚ carbonate and bicarbonate‚ by using a potentiometric titration. We can determine the concentration of the bases in the reactions ( H+ +CO3- < ==> HCO3- and H+ + HCO3- < ==> H2CO3-) by the way the pH of the solution changes. The way the pH changes when a strong acid is added can
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