Experiment 2: Estimation of protein concentration Name: Wong Jiun Hao ID: 00000011136 Cohort: BM114 Module: Biological Science Table of Contents Title 1 Table of Contents 2 Introduction 3 Objectives 4 Procedure 4 Results 5-6 Discussion 7-8 Conclusion 9 References 9 Introduction The concentration of protein in a sample can be obtained by using the Bradford protein assay method. The method requires a spectrophotometer
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Protein and Amino Acid Supplementation in Sports The popularity of increasing the “performance-enhancing” supplements‚ Protein and amino acids‚ has flourished among all athletes. This increase is attributed to the belief by many of the athletes that it provides endurance‚ strength and speed enhancement. Amino Acids (AA) enhanced physical feats‚ improved energy and recovery sooner from fatigue. The three vital Amino Acids which were given a high focal point among athletes are leucine‚ isoleucine
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The Plant Cell‚ Vol. 17‚ 1829–1838‚ June 2005‚ www.plantcell.org ª 2005 American Society of Plant Biologists Dual Role for Tomato Heat Shock Protein 21: Protecting Photosystem II from Oxidative Stress and Promoting Color Changes during Fruit Maturation Inbal Neta-Sharir‚a Tal Isaacson‚b Susan Lurie‚c and David Weissa‚1 a Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture‚ Faculty of Agricultural‚ Food‚ and Environmental Quality Sciences‚ Hebrew University of Jerusalem‚ Rehovot
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Green Leaves Transport Ltd. Green Leaves Transport Ltd. In the exercise of their ownership rights‚ they should: - Configure their company as an instrument at the service of creating wealth‚ making their indisputable objective of obtaining a profit compatible with sustainable‚ environmentally sound social development‚ making certain that all activities are carried out in an ethical and responsible manner. - Configure the company as a medium and long-term entity‚ not compromising its
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of diet an organism is on. For example‚ a diet high in fat would result in a low R.Q due to the greater amount of O2 molecules required to metabolize that fat for ATP. On the contrary‚ a high protein diet would result in a larger R.Q due to a fewer amount of O2 molecules required to metabolize that protein for ATP. In a study performed by Renault et al. (2003) it was found that starvation resulted in a significant decrease in metabolic rate in the pest beetle Alphitobius diaperinus whereas the
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2 Production of Protein from Cloned Genes Now that we have covered the basic techniques involved in gene cloning and DNA analysis and examined how these techniques are used in research‚ we can move on to consider how recombinant DNA technology is being applied in biotechnology. This is not a new subject‚ although biotechnology has received far more attention during recent years than it ever has in the past. Biotechnology can be defined as the use of biological processes in industry and technology
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Eddie Lai Clark 7 12/15/11 Cell Division/ DNA / Protein Synthesis Study guide AA: Simple definition AA: Simple explanation AA: Detailed explanation/drawing AA: Questions 1. What is transformation? * Movement of a gene from one organism to another 2. What did Griffith show? * Showed either protein or DNA causes transformation 3. What did Avery show? * Showed that DNA causes transformation or that DNA is hereditary material 4. What did Hershey & Chase show
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Specificity of Protein-Ligand Binding 1). One can conclude that Orange G. has the lowest affinity for the albumin. In the experiment the concentration of Orange G that binds to protein was a lot less than either Ponceau S. or Bromophenol blue. The ligand with the highest affinity to BSA was a little more difficult to decipher. The experiment shows that all of the 2 µL of both Ponceau S. and Bromophenol blue bind to the BSA. However‚ when 5 µL of the ligand is added the BSA is saturated and cannot
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In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
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Protein Article Research SCI/241 September 27‚ 2012 Roberta Harbison Protein Article Research Protein is an essential part of a healthy diet. It is made of different combinations of 20 different amino acids. Protein is found in every cell‚ tissue‚ and organ of the body. It is important to consume an adequate amount of protein because our bodies are constantly using the protein we digest to make more amino acids. Some amino acids cannot be produced by the body and it is essential that
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